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- W1987293717 abstract "The use of thermally forme pH gradients permits macromolecular separations in open-channel isoelectric focusing (IEF), simplifying product recovery, shortening run times and eliminating costly carrier ampholytes. Because of these unique advantages and other limitations this IEF method may effect purifications on a preparative scale more effectively than on an analytical scale where other IEF methods may cover a larger pH range. Heat exchangers at both ends of a 3.18 mm × 5.0 mm × 28.0 mm channel generated the temperature gradient (4°C cm−1). Owing to the large temperature dependence of the pH (dpH/dT= −0.028 K−1), the Tris—HCl buffer formed the pH gradient in the free solution. In a 25 V cm−1 electric field, a dilute mixture of hemoglobin variants A and S (ca. 0.8 mg mg−1, isoelectric points 6.98 and 7.2, respectively) were separated and concentrated by a factor of 20." @default.
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- W1987293717 date "1989-01-01" @default.
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- W1987293717 title "Open-channel isolectric focusing in thermally engendered pH gradients" @default.
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- W1987293717 doi "https://doi.org/10.1016/s0021-9673(01)84298-9" @default.
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