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- W1987295564 abstract "Covalently closed circular Col E1 DNA was ultraviolet-irradiated with a dose of 60 J/m2, thus introducing about 3.2 pyrimidine dimers per DNA molecule. Treatment of irradiated Col E1 DNA with Micrococcus luteus correndonuclease resulted, in the vicinity of pyrimidine dimers, in an average of 3.3 incisions per DNA molecule, and converted DNA to the open circular form. Incised Col E1 DNA stimulated no reaction with calf thymus DNA polymerase α but was recognized as a template by DNA polymerase β. The latter enzyme incorporated about 1.6 molecules of dTMP (corresponding to 6 molecules of dNMP) per one correndonuclease incision. The length of the DNA polymerase β product was comparable to the anticipated length of the DNA region within which the hydrogen bonds were disrupted owing to dimer formation. The enzyme required Mg2+ and four dNTPs for reaction and was resistant to N-ethylmaleimide or p-mercuribenzoate." @default.
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- W1987295564 date "1980-09-01" @default.
- W1987295564 modified "2023-09-26" @default.
- W1987295564 title "The DNA polymerase β reaction with ultraviolet-irradiated DNA incised by correndonuclease" @default.
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- W1987295564 doi "https://doi.org/10.1016/0005-2787(80)90235-x" @default.
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