FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987316059> ?p ?o ?g. }

• W1987316059 endingPage "1771" @default.
• W1987316059 startingPage "1763" @default.
• W1987316059 abstract "Retinal disease is the major cause of irreversible blindness in developed countries. Transplantation of photoreceptor precursor cells (PPCs) derived from human embryonic stem cells (hESCs) is a promising and widely applicable approach for the treatment of these blinding conditions. Previously, it has been shown that after transplantation into the degenerating retina, the percentage of PPCs that undergo functional integration is low. The factors that inhibit PPC engraftment remain largely unknown, in part, because so many adverse factors could be at play during in vivo experiments. To advance our knowledge in overcoming potential adverse effects and optimize PPC transplantation, we have developed a novel ex vivo system. Harvested neural retina was placed directly on top of cultured retinal pigment epithelial (RPE) cells from a number of different sources. To mimic PPC transplantation into the subretinal space, hESC-derived PPCs were inserted between the retinal explant and underlying RPE. Explants cocultured with hESC-derived RPE maintained normal gross morphology and viability for up to 2 weeks, whereas the explants cultured on ARPE19 and RPE-J failed by 7 days. Furthermore, the proportion of PPCs expressing ribbon synapse-specific proteins BASSOON and RIBEYE was significantly higher when cocultured with hESC-derived RPE (20% and 10%, respectively), than when cocultured with ARPE19 (only 6% and 2%, respectively). In the presence of the synaptogenic factor thrombospondin-1 (TSP-1), the proportion of BASSOON-positive and RIBEYE-positive PPCs cocultured with hESC-derived RPE increased to ∼30% and 15%, respectively. These data demonstrate the utility of an ex vivo model system to define factors, such as TSP-1, which could influence integration efficiency in future in vivo experiments in models of retinal degeneration." @default.
• W1987316059 created "2016-06-24" @default.
• W1987316059 creator A5016303561 @default.
• W1987316059 creator A5040970112 @default.
• W1987316059 creator A5051291616 @default.
• W1987316059 creator A5069203405 @default.
• W1987316059 creator A5081585732 @default.
• W1987316059 date "2015-06-01" @default.
• W1987316059 modified "2023-10-13" @default.
• W1987316059 title "Enhanced Functional Integration of Human Photoreceptor Precursors into Human and Rodent Retina in an<i>Ex Vivo</i>Retinal Explant Model System" @default.
• W1987316059 cites W1483496013 @default.
• W1987316059 cites W1593266928 @default.
• W1987316059 cites W1963817246 @default.
• W1987316059 cites W1966040258 @default.
• W1987316059 cites W1966300591 @default.
• W1987316059 cites W1968011780 @default.
• W1987316059 cites W1968078502 @default.
• W1987316059 cites W1976301870 @default.
• W1987316059 cites W1978731296 @default.
• W1987316059 cites W1988155168 @default.
• W1987316059 cites W1997059254 @default.
• W1987316059 cites W1997480136 @default.
• W1987316059 cites W2011634069 @default.
• W1987316059 cites W2013433258 @default.
• W1987316059 cites W2013617404 @default.
• W1987316059 cites W2019586013 @default.
• W1987316059 cites W2021839551 @default.
• W1987316059 cites W2023339971 @default.
• W1987316059 cites W2034908194 @default.
• W1987316059 cites W2039131749 @default.
• W1987316059 cites W2040434386 @default.
• W1987316059 cites W2044092807 @default.
• W1987316059 cites W2047393488 @default.
• W1987316059 cites W2048140742 @default.
• W1987316059 cites W2048338614 @default.
• W1987316059 cites W2073947789 @default.
• W1987316059 cites W2077480943 @default.
• W1987316059 cites W2081829492 @default.
• W1987316059 cites W2088623488 @default.
• W1987316059 cites W2091315047 @default.
• W1987316059 cites W2092167465 @default.
• W1987316059 cites W2098622411 @default.
• W1987316059 cites W2100861163 @default.
• W1987316059 cites W2107277218 @default.
• W1987316059 cites W2109583860 @default.
• W1987316059 cites W2118720693 @default.
• W1987316059 cites W2128803651 @default.
• W1987316059 cites W2142080905 @default.
• W1987316059 cites W2168408556 @default.
• W1987316059 cites W2192080449 @default.
• W1987316059 cites W2410313142 @default.
• W1987316059 cites W2466947516 @default.
• W1987316059 cites W4249994679 @default.
• W1987316059 doi "https://doi.org/10.1089/ten.tea.2014.0669" @default.
• W1987316059 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4449699" @default.
• W1987316059 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25693608" @default.
• W1987316059 hasPublicationYear "2015" @default.
• W1987316059 type Work @default.
• W1987316059 sameAs 1987316059 @default.
• W1987316059 citedByCount "13" @default.
• W1987316059 countsByYear W19873160592016 @default.
• W1987316059 countsByYear W19873160592017 @default.
• W1987316059 countsByYear W19873160592018 @default.
• W1987316059 countsByYear W19873160592019 @default.
• W1987316059 countsByYear W19873160592020 @default.
• W1987316059 crossrefType "journal-article" @default.
• W1987316059 hasAuthorship W1987316059A5016303561 @default.
• W1987316059 hasAuthorship W1987316059A5040970112 @default.
• W1987316059 hasAuthorship W1987316059A5051291616 @default.
• W1987316059 hasAuthorship W1987316059A5069203405 @default.
• W1987316059 hasAuthorship W1987316059A5081585732 @default.
• W1987316059 hasBestOaLocation W19873160592 @default.
• W1987316059 hasConcept C104317684 @default.
• W1987316059 hasConcept C105702510 @default.
• W1987316059 hasConcept C141071460 @default.
• W1987316059 hasConcept C145103041 @default.
• W1987316059 hasConcept C150903083 @default.
• W1987316059 hasConcept C157890192 @default.
• W1987316059 hasConcept C169760540 @default.
• W1987316059 hasConcept C202751555 @default.
• W1987316059 hasConcept C207001950 @default.
• W1987316059 hasConcept C26291073 @default.
• W1987316059 hasConcept C2777093970 @default.
• W1987316059 hasConcept C2778024200 @default.
• W1987316059 hasConcept C2780827179 @default.
• W1987316059 hasConcept C2781114197 @default.
• W1987316059 hasConcept C2911091166 @default.
• W1987316059 hasConcept C55493867 @default.
• W1987316059 hasConcept C71924100 @default.
• W1987316059 hasConcept C86803240 @default.
• W1987316059 hasConcept C95444343 @default.
• W1987316059 hasConceptScore W1987316059C104317684 @default.
• W1987316059 hasConceptScore W1987316059C105702510 @default.
• W1987316059 hasConceptScore W1987316059C141071460 @default.
• W1987316059 hasConceptScore W1987316059C145103041 @default.
• W1987316059 hasConceptScore W1987316059C150903083 @default.
• W1987316059 hasConceptScore W1987316059C157890192 @default.
• W1987316059 hasConceptScore W1987316059C169760540 @default.

• next