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• W1987321824 abstract "In order to improve the production of human-like collagen III (HLC III) by fed-batch culture of recombinant Escherichia coli BL21, the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters. Three variables (induction time, inoculum age and pH), which have significant effects on HLC III production, were selected from eight variables by Plackett-Burman design. With the regression coefficient analysis in the Box-Behnken design, a relationship between HLC III production and three significant factors was obtained, and the optimum levels of the three variables were as follows: induction time 3.2h, inoculum age 12.6 h and pH 6.7. The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant. An average 9.68 g·L−1HLC III production was attained in the validation experiment under optimized condition, which was 80% higher than the yield of 5.36 g·L−1 before optimization." @default.
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• W1987321824 date "2010-02-01" @default.
• W1987321824 modified "2023-09-23" @default.
• W1987321824 title "Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology" @default.
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• W1987321824 doi "https://doi.org/10.1016/s1004-9541(08)60334-1" @default.
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