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• W1987336883 endingPage "694" @default.
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• W1987336883 abstract "Mycobacterium avium complex (MAC) currently comprises eight species of environmental and animal-associated, slowly-growing mycobacteria: Mycobacterium avium , Mycobacterium intracellulare , Mycobacterium chimaera , Mycobacterium colombiense , Mycobacterium arosiense , Mycobacterium bouchedurhonense , Mycobacterium marseillense and Mycobacterium timonense . In humans, MAC organisms are responsible for opportunistic infections whose unique epidemiology remains poorly understood, in part due to the lack of a genotyping method applicable to all eight MAC species. In this study we developed multispacer sequence typing (MST), a sequencing-based method, for the genotyping of MAC organisms. An alignment of the genome sequence of M. avium subsp. hominissuis strain104 and M. avium subsp. paratuberculosis strain K-10 revealed621 intergenic spacers <1000 bp. From these, 16 spacers were selected that ranged from 300 to 800 bp and contained a number of variable bases, <50 within each of the 16 spacers. Four spacers were successfully PCR-amplified and sequenced in 11 reference strains. Combining the sequence of these four spacers in 106 MAC organisms, including 83 M. avium , 11 M. intracellulare , six M. chimaera , two M. colombiense and one each of M. arosiense , M. bouchedurhonense , M. marseillense and M. timonense , yielded a total of 45 spacer types, with an index of discrimination of 0.94. Each spacer type was specific for a species and certain spacer types were specific for subspecies of M. avium . MST is a new method for genotyping of organisms belonging to any one of the eight MAC species tested in this study." @default.
• W1987336883 created "2016-06-24" @default.
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• W1987336883 date "2010-03-01" @default.
• W1987336883 modified "2023-09-25" @default.
• W1987336883 title "Genotyping of Mycobacterium avium complex organisms using multispacer sequence typing" @default.
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• W1987336883 doi "https://doi.org/10.1099/mic.0.033522-0" @default.
• W1987336883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19926652" @default.
• W1987336883 hasPublicationYear "2010" @default.
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