FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987348669> ?p ?o ?g. }

• W1987348669 endingPage "305" @default.
• W1987348669 startingPage "296" @default.
• W1987348669 abstract "Accurate real-time measurements of proton concentration gradients are pivotal to mechanistic studies of proton translocation by membrane-bound enzymes. Here we report a detailed characterization of the pH-sensitive fluorescent nanoprobe Glu(3), which is well suited for pH measurements in microcompartmentalized biological systems. The probe is a polyglutamic porphyrin dendrimer in which multiple carboxylate termini ensure its high water solubility and prevent its diffusion across phospholipid membranes. The probe's pK is in the physiological pH range, and its protonation can be followed ratiometrically by absorbance or fluorescence in the ultraviolet-visible spectral region. The usefulness of the probe was enhanced by using a semiautomatic titration system coupled to a charge-coupled device (CCD) spectrometer, enabling fast and accurate titrations and full spectral coverage of the system at millisecond time resolution. The probe's pK was measured in bulk solutions as well as inside large unilamellar vesicles in the presence of physiologically relevant ions. Glu(3) was found to be completely membrane impermeable, and its distinct spectroscopic features permit pH measurements inside closed membrane vesicles, enabling quantitative mechanistic studies of membrane-spanning proteins. Performance of the probe was demonstrated by monitoring the rate of proton leakage through the phospholipid bilayer in large vesicles with and without the uncoupler gramicidin present. Overall, as a probe for biological proton translocation measurements, Glu(3) was found to be superior to the commercially available pH indicators." @default.
• W1987348669 created "2016-06-24" @default.
• W1987348669 creator A5005833302 @default.
• W1987348669 creator A5010869647 @default.
• W1987348669 creator A5078310329 @default.
• W1987348669 creator A5084667084 @default.
• W1987348669 creator A5085548406 @default.
• W1987348669 creator A5091754360 @default.
• W1987348669 date "2009-05-01" @default.
• W1987348669 modified "2023-10-12" @default.
• W1987348669 title "Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes" @default.
• W1987348669 cites W136984087 @default.
• W1987348669 cites W1536874112 @default.
• W1987348669 cites W1543955271 @default.
• W1987348669 cites W1554135729 @default.
• W1987348669 cites W1554459256 @default.
• W1987348669 cites W1662391448 @default.
• W1987348669 cites W1761602676 @default.
• W1987348669 cites W1966000480 @default.
• W1987348669 cites W1969205203 @default.
• W1987348669 cites W1970049852 @default.
• W1987348669 cites W1971389338 @default.
• W1987348669 cites W1973432768 @default.
• W1987348669 cites W1976798586 @default.
• W1987348669 cites W1984343393 @default.
• W1987348669 cites W1988312683 @default.
• W1987348669 cites W1991009742 @default.
• W1987348669 cites W1993532849 @default.
• W1987348669 cites W1993863299 @default.
• W1987348669 cites W2007986803 @default.
• W1987348669 cites W2016486229 @default.
• W1987348669 cites W2022395635 @default.
• W1987348669 cites W2024813557 @default.
• W1987348669 cites W2049245912 @default.
• W1987348669 cites W2056467918 @default.
• W1987348669 cites W2065476796 @default.
• W1987348669 cites W2068476566 @default.
• W1987348669 cites W2070960441 @default.
• W1987348669 cites W2076875995 @default.
• W1987348669 cites W2083258595 @default.
• W1987348669 cites W2085946296 @default.
• W1987348669 cites W2096381222 @default.
• W1987348669 cites W2096535122 @default.
• W1987348669 cites W2100317745 @default.
• W1987348669 cites W2103972113 @default.
• W1987348669 cites W2106490309 @default.
• W1987348669 cites W2106973449 @default.
• W1987348669 cites W2111682630 @default.
• W1987348669 cites W2149670205 @default.
• W1987348669 cites W2151985831 @default.
• W1987348669 cites W2165650569 @default.
• W1987348669 doi "https://doi.org/10.1016/j.ab.2009.02.023" @default.
• W1987348669 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2804896" @default.
• W1987348669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19248752" @default.
• W1987348669 hasPublicationYear "2009" @default.
• W1987348669 type Work @default.
• W1987348669 sameAs 1987348669 @default.
• W1987348669 citedByCount "22" @default.
• W1987348669 countsByYear W19873486692013 @default.
• W1987348669 countsByYear W19873486692014 @default.
• W1987348669 countsByYear W19873486692015 @default.
• W1987348669 countsByYear W19873486692017 @default.
• W1987348669 countsByYear W19873486692018 @default.
• W1987348669 countsByYear W19873486692019 @default.
• W1987348669 countsByYear W19873486692020 @default.
• W1987348669 countsByYear W19873486692023 @default.
• W1987348669 crossrefType "journal-article" @default.
• W1987348669 hasAuthorship W1987348669A5005833302 @default.
• W1987348669 hasAuthorship W1987348669A5010869647 @default.
• W1987348669 hasAuthorship W1987348669A5078310329 @default.
• W1987348669 hasAuthorship W1987348669A5084667084 @default.
• W1987348669 hasAuthorship W1987348669A5085548406 @default.
• W1987348669 hasAuthorship W1987348669A5091754360 @default.
• W1987348669 hasBestOaLocation W19873486692 @default.
• W1987348669 hasConcept C113196181 @default.
• W1987348669 hasConcept C121332964 @default.
• W1987348669 hasConcept C130316041 @default.
• W1987348669 hasConcept C178790620 @default.
• W1987348669 hasConcept C179104552 @default.
• W1987348669 hasConcept C184866935 @default.
• W1987348669 hasConcept C185592680 @default.
• W1987348669 hasConcept C202800777 @default.
• W1987348669 hasConcept C2778001640 @default.
• W1987348669 hasConcept C2779179816 @default.
• W1987348669 hasConcept C2779480358 @default.
• W1987348669 hasConcept C2992012372 @default.
• W1987348669 hasConcept C39944091 @default.
• W1987348669 hasConcept C41625074 @default.
• W1987348669 hasConcept C43617362 @default.
• W1987348669 hasConcept C55493867 @default.
• W1987348669 hasConcept C62520636 @default.
• W1987348669 hasConcept C75473681 @default.
• W1987348669 hasConcept C91881484 @default.
• W1987348669 hasConceptScore W1987348669C113196181 @default.
• W1987348669 hasConceptScore W1987348669C121332964 @default.
• W1987348669 hasConceptScore W1987348669C130316041 @default.
• W1987348669 hasConceptScore W1987348669C178790620 @default.
• W1987348669 hasConceptScore W1987348669C179104552 @default.

• next