FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987357029> ?p ?o ?g. }

• W1987357029 abstract "Knowledge about signaling pathways is typically compiled based on data gathered using different cell lines. This approach implicitly assumes that the cell line dependence is not important. However, different cell lines do not always respond to a particular stimulus in the same way, and lack of coherent data collected from closely related cellular systems can be detrimental to the efforts to understand the regulation of biological processes. To address this issue, we created a clone library of human mammary epithelial (HME) cells that expresses different levels of HER2 and HER3 receptors in combination with endogenous EGFR/HER1. Using our clone library, we have quantified the receptor activation patterns and systematically tested the validity of the existing hypotheses about the interaction patterns between HER1-3 receptors.Our study identified HER2 as the dominant dimerization partner for both EGFR and HER3. Contrary to earlier suggestions, we find that lateral interactions with HER2 do not lead to strong transactivation between EGFR and HER3, i.e., EGFR activation and HER3 activation are only weakly linked in HME cells. We also find that observed weak transactivation is uni-directional where stimulation of EGFR leads to HER3 activation whereas HER3 stimulation does not activate the EGFR. Repeating our experiments at lower cell confluency established that cell confluency is not a major factor in the observed interaction patterns. We have also quantified the dependence of the kinetics of Erk and Akt activation on different HER receptors. We found that HER3 signaling makes the strongest contribution to Akt activation and that, stimulation of either EGFR or HER3 leads to significant Erk activation.Our study shows that clone cell libraries can be a powerful resource in systems biology research by making it possible to differentiate between various hypotheses in a consistent cellular background. Using our constructed clone library we profiled the cell signaling patterns to establish the role of HER2 in the crosstalk between EGFR and HER3 receptors in HME cells. Our results for HME cells show that the weak linkage between EGFR and HER3 pathways can lead to distinct downstream cellular signaling patterns in response to the ligands of these two receptors." @default.
• W1987357029 created "2016-06-24" @default.
• W1987357029 creator A5010647553 @default.
• W1987357029 creator A5013793470 @default.
• W1987357029 creator A5042451458 @default.
• W1987357029 creator A5044544424 @default.
• W1987357029 creator A5061290912 @default.
• W1987357029 creator A5089763500 @default.
• W1987357029 date "2009-10-31" @default.
• W1987357029 modified "2023-10-18" @default.
• W1987357029 title "HER/ErbB receptor interactions and signaling patterns in human mammary epithelial cells" @default.
• W1987357029 cites W136642420 @default.
• W1987357029 cites W1870193477 @default.
• W1987357029 cites W1964321121 @default.
• W1987357029 cites W1969937534 @default.
• W1987357029 cites W1971727526 @default.
• W1987357029 cites W1975949792 @default.
• W1987357029 cites W1982675645 @default.
• W1987357029 cites W1986335023 @default.
• W1987357029 cites W1995819749 @default.
• W1987357029 cites W1998809668 @default.
• W1987357029 cites W1998971866 @default.
• W1987357029 cites W2001627828 @default.
• W1987357029 cites W2004490932 @default.
• W1987357029 cites W2006752099 @default.
• W1987357029 cites W2007022455 @default.
• W1987357029 cites W2007649418 @default.
• W1987357029 cites W2008021119 @default.
• W1987357029 cites W2014111401 @default.
• W1987357029 cites W2014457594 @default.
• W1987357029 cites W2014476931 @default.
• W1987357029 cites W2017081079 @default.
• W1987357029 cites W2017397076 @default.
• W1987357029 cites W2022406746 @default.
• W1987357029 cites W2025561101 @default.
• W1987357029 cites W2026394641 @default.
• W1987357029 cites W2029999856 @default.
• W1987357029 cites W2031016321 @default.
• W1987357029 cites W2036253797 @default.
• W1987357029 cites W2040142253 @default.
• W1987357029 cites W2042313347 @default.
• W1987357029 cites W2043911313 @default.
• W1987357029 cites W2057066413 @default.
• W1987357029 cites W2058645214 @default.
• W1987357029 cites W2072077210 @default.
• W1987357029 cites W2076105533 @default.
• W1987357029 cites W2076797981 @default.
• W1987357029 cites W2083540678 @default.
• W1987357029 cites W2083831897 @default.
• W1987357029 cites W2087846936 @default.
• W1987357029 cites W2090855460 @default.
• W1987357029 cites W2091017815 @default.
• W1987357029 cites W2091987058 @default.
• W1987357029 cites W2093868559 @default.
• W1987357029 cites W2096287391 @default.
• W1987357029 cites W2096595244 @default.
• W1987357029 cites W2097097666 @default.
• W1987357029 cites W2108246579 @default.
• W1987357029 cites W2122003447 @default.
• W1987357029 cites W2124542546 @default.
• W1987357029 cites W2126841263 @default.
• W1987357029 cites W2130840817 @default.
• W1987357029 cites W2133663537 @default.
• W1987357029 cites W2134477837 @default.
• W1987357029 cites W2141167338 @default.
• W1987357029 cites W2143651667 @default.
• W1987357029 cites W2165003903 @default.
• W1987357029 cites W2166609912 @default.
• W1987357029 cites W2182962878 @default.
• W1987357029 cites W2326198238 @default.
• W1987357029 cites W2329126676 @default.
• W1987357029 cites W65096997 @default.
• W1987357029 doi "https://doi.org/10.1186/1471-2121-10-78" @default.
• W1987357029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2776588" @default.
• W1987357029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19878579" @default.
• W1987357029 hasPublicationYear "2009" @default.
• W1987357029 type Work @default.
• W1987357029 sameAs 1987357029 @default.
• W1987357029 citedByCount "37" @default.
• W1987357029 countsByYear W19873570292012 @default.
• W1987357029 countsByYear W19873570292013 @default.
• W1987357029 countsByYear W19873570292014 @default.
• W1987357029 countsByYear W19873570292015 @default.
• W1987357029 countsByYear W19873570292016 @default.
• W1987357029 countsByYear W19873570292017 @default.
• W1987357029 countsByYear W19873570292018 @default.
• W1987357029 countsByYear W19873570292019 @default.
• W1987357029 countsByYear W19873570292020 @default.
• W1987357029 crossrefType "journal-article" @default.
• W1987357029 hasAuthorship W1987357029A5010647553 @default.
• W1987357029 hasAuthorship W1987357029A5013793470 @default.
• W1987357029 hasAuthorship W1987357029A5042451458 @default.
• W1987357029 hasAuthorship W1987357029A5044544424 @default.
• W1987357029 hasAuthorship W1987357029A5061290912 @default.
• W1987357029 hasAuthorship W1987357029A5089763500 @default.
• W1987357029 hasBestOaLocation W19873570291 @default.
• W1987357029 hasConcept C104317684 @default.
• W1987357029 hasConcept C128240485 @default.
• W1987357029 hasConcept C1292079 @default.
• W1987357029 hasConcept C169760540 @default.

• next