FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987360507> ?p ?o ?g. }

• W1987360507 endingPage "6779" @default.
• W1987360507 startingPage "6775" @default.
• W1987360507 abstract "Thrombin is a serine protease that acts as a procoagulant by clotting fibrinogen and activating platelets and as an anticoagulant by activating protein C in a thrombomodulin-dependent reaction. Fibrinogen and thrombomodulin bind competitively to an anion-binding exosite on thrombin. We prepared recombinant normal human thrombin and mutant thrombins with single amino acid substitutions in order to localize and distinguish the fibrinogen- and thrombomodulin-binding sites. Normal and mutant thrombins had similar amidolytic activity. Thrombin K52E had approximately 2.5-fold increased protein C-activating activity but only approximately 17% of normal fibrinogen-clotting activity. Thrombin R70E had normal fibrinogen-clotting activity but only approximately 7% of normal protein C-activating activity. Thrombin R68E had markedly reduced activity in both assays. Decreased activation of protein C correlated with decreased binding affinity for thrombomodulin, and ability to activate platelets correlated directly with fibrinogen-clotting activity. These results demonstrate that thrombins with predominantly anticoagulant or procoagulant activity can be created by mutagenesis and that thrombomodulin- and fibrinogen-binding sites on thrombin may overlap but are not identical." @default.
• W1987360507 created "2016-06-24" @default.
• W1987360507 creator A5005704032 @default.
• W1987360507 creator A5021544303 @default.
• W1987360507 creator A5046167047 @default.
• W1987360507 creator A5060327428 @default.
• W1987360507 creator A5061354837 @default.
• W1987360507 creator A5070640404 @default.
• W1987360507 date "1991-08-01" @default.
• W1987360507 modified "2023-09-30" @default.
• W1987360507 title "Single amino acid substitutions dissociate fibrinogen-clotting and thrombomodulin-binding activities of human thrombin." @default.
• W1987360507 cites W107781538 @default.
• W1987360507 cites W1243769774 @default.
• W1987360507 cites W1517385423 @default.
• W1987360507 cites W1518197977 @default.
• W1987360507 cites W1604149400 @default.
• W1987360507 cites W1608374437 @default.
• W1987360507 cites W1665121191 @default.
• W1987360507 cites W1965372133 @default.
• W1987360507 cites W1969347289 @default.
• W1987360507 cites W1991330699 @default.
• W1987360507 cites W2018235565 @default.
• W1987360507 cites W2018289835 @default.
• W1987360507 cites W2032118018 @default.
• W1987360507 cites W2038058775 @default.
• W1987360507 cites W2042526837 @default.
• W1987360507 cites W2054687894 @default.
• W1987360507 cites W2059998986 @default.
• W1987360507 cites W2072730036 @default.
• W1987360507 cites W2074044112 @default.
• W1987360507 cites W2077182436 @default.
• W1987360507 cites W2081753893 @default.
• W1987360507 cites W2100837269 @default.
• W1987360507 cites W2100851792 @default.
• W1987360507 cites W2113208052 @default.
• W1987360507 cites W2118929543 @default.
• W1987360507 cites W2121542842 @default.
• W1987360507 cites W2141373630 @default.
• W1987360507 cites W2395791699 @default.
• W1987360507 cites W4230338168 @default.
• W1987360507 doi "https://doi.org/10.1073/pnas.88.15.6775" @default.
• W1987360507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/52171" @default.
• W1987360507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1650482" @default.
• W1987360507 hasPublicationYear "1991" @default.
• W1987360507 type Work @default.
• W1987360507 sameAs 1987360507 @default.
• W1987360507 citedByCount "125" @default.
• W1987360507 countsByYear W19873605072012 @default.
• W1987360507 countsByYear W19873605072013 @default.
• W1987360507 countsByYear W19873605072014 @default.
• W1987360507 countsByYear W19873605072015 @default.
• W1987360507 countsByYear W19873605072016 @default.
• W1987360507 countsByYear W19873605072017 @default.
• W1987360507 countsByYear W19873605072018 @default.
• W1987360507 countsByYear W19873605072019 @default.
• W1987360507 countsByYear W19873605072020 @default.
• W1987360507 countsByYear W19873605072023 @default.
• W1987360507 crossrefType "journal-article" @default.
• W1987360507 hasAuthorship W1987360507A5005704032 @default.
• W1987360507 hasAuthorship W1987360507A5021544303 @default.
• W1987360507 hasAuthorship W1987360507A5046167047 @default.
• W1987360507 hasAuthorship W1987360507A5060327428 @default.
• W1987360507 hasAuthorship W1987360507A5061354837 @default.
• W1987360507 hasAuthorship W1987360507A5070640404 @default.
• W1987360507 hasBestOaLocation W19873605072 @default.
• W1987360507 hasConcept C126322002 @default.
• W1987360507 hasConcept C153911025 @default.
• W1987360507 hasConcept C185592680 @default.
• W1987360507 hasConcept C201267052 @default.
• W1987360507 hasConcept C203014093 @default.
• W1987360507 hasConcept C2777292125 @default.
• W1987360507 hasConcept C2778382381 @default.
• W1987360507 hasConcept C2779036427 @default.
• W1987360507 hasConcept C2781287897 @default.
• W1987360507 hasConcept C55493867 @default.
• W1987360507 hasConcept C71924100 @default.
• W1987360507 hasConcept C86803240 @default.
• W1987360507 hasConcept C89560881 @default.
• W1987360507 hasConceptScore W1987360507C126322002 @default.
• W1987360507 hasConceptScore W1987360507C153911025 @default.
• W1987360507 hasConceptScore W1987360507C185592680 @default.
• W1987360507 hasConceptScore W1987360507C201267052 @default.
• W1987360507 hasConceptScore W1987360507C203014093 @default.
• W1987360507 hasConceptScore W1987360507C2777292125 @default.
• W1987360507 hasConceptScore W1987360507C2778382381 @default.
• W1987360507 hasConceptScore W1987360507C2779036427 @default.
• W1987360507 hasConceptScore W1987360507C2781287897 @default.
• W1987360507 hasConceptScore W1987360507C55493867 @default.
• W1987360507 hasConceptScore W1987360507C71924100 @default.
• W1987360507 hasConceptScore W1987360507C86803240 @default.
• W1987360507 hasConceptScore W1987360507C89560881 @default.
• W1987360507 hasIssue "15" @default.
• W1987360507 hasLocation W19873605071 @default.
• W1987360507 hasLocation W19873605072 @default.
• W1987360507 hasLocation W19873605073 @default.
• W1987360507 hasLocation W19873605074 @default.
• W1987360507 hasOpenAccess W1987360507 @default.
• W1987360507 hasPrimaryLocation W19873605071 @default.

• next