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- W1987408740 abstract "This paper presents an intensity normalization method for analysis of liver tissue images, acquired using the two-photonmicroscopy system at different stages of fibrosis. Image informatics methods require precise intensity segmentation foranalysis of collagen, vessel and cellular structures. Intensities of the images recorded at different time intervalscorresponding to the progression of fibrosis could vary spatially and temporally depending on the experimentalconditions. These variations significantly affect the image segmentation process and thus the final image analysis,especially when automatic computer-based methods are used for diagnostic parameters quantification. We propose anadaptive intensity normalization method that facilitates spatial and temporal intensity variations of the images before thesegmentation process. The images are first portioned into a tessellation of regions with relatively uniform backgroundpixels intensities and then the normalization is performed to make sure the intensity range is unified throughout thewhole set of image data. This approach is further extended for montage of images acquired from multianodephotomultiplier tube based multifocal multiphoton microscope (MMM) system. The proposed approach significantlyimproves the automated analysis of images with varying intensities without any user intervention." @default.
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- W1987408740 date "2011-02-10" @default.
- W1987408740 modified "2023-10-18" @default.
- W1987408740 title "Intensity normalization of two-photon microscopy images for liver fibrosis analysis" @default.
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- W1987408740 doi "https://doi.org/10.1117/12.876246" @default.
- W1987408740 hasPublicationYear "2011" @default.
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