FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987418111> ?p ?o ?g. }

• W1987418111 endingPage "1691" @default.
• W1987418111 startingPage "1682" @default.
• W1987418111 abstract "ABSTRACT Seven distinct Bacillus thuringiensis subsp. aizawai integrants were constructed that carried the chitinase ( chiBlA ) gene from B. licheniformis under the control of the cry11Aa promoter and terminator with and without p19 and p20 genes. The toxicity of B. thuringiensis subsp. aizawai integrants against second-instar Spodoptera litura larvae was increased 1.8- to 4.6-fold compared to that of the wild-type strain (BTA1). Surprisingly, the enhanced toxicity in some strains of B. thuringiensis subsp. aizawai integrants ( Bta P19CS, Bta P19CSter, and Bta CAT) correlated with an increase in toxin formation. To investigate the role of these genes in toxin production, the expression profiles of the toxin genes, cry1Aa and chiBlA , as well as their transcriptional regulators ( sigK and sigE ), were analyzed by quantitative real-time RT-PCR (qPCR) from BTA1, Bta P19CS, and Bta CAT. Expression levels of cry1Aa in these two integrants increased about 2- to 3-fold compared to those of BTA1. The expression of the transcription factor sigK also was prolonged in the integrants compared to that of the wild type; however, sigE expression was unchanged. Western blot analysis of σ E and σ K showed the prolonged accumulation of σ E in the integrants compared to that of BTA1, resulting in the increased synthesis of pro-σ K up to T 17 after the onset of sporulation in both Bta P19CS and Bta CAT compared to that of T 13 in BTA1. The results from qPCR indicate clearly that the cry1Aa promoter activity was influenced most strongly by σ E , whereas cry11Aa depended mostly on σ K . These results on large-crystal toxin formation with enhanced toxicity should provide useful information for the generation of strains with improved insecticidal activity." @default.
• W1987418111 created "2016-06-24" @default.
• W1987418111 creator A5002845881 @default.
• W1987418111 creator A5024504306 @default.
• W1987418111 date "2012-03-15" @default.
• W1987418111 modified "2023-09-25" @default.
• W1987418111 title "Large Crystal Toxin Formation in Chromosomally Engineered Bacillus thuringiensis subsp. aizawai Due to σ E Accumulation" @default.
• W1987418111 cites W1489551586 @default.
• W1987418111 cites W1518452118 @default.
• W1987418111 cites W1535002104 @default.
• W1987418111 cites W1548162677 @default.
• W1987418111 cites W1558790011 @default.
• W1987418111 cites W1858120728 @default.
• W1987418111 cites W1860006701 @default.
• W1987418111 cites W1897953158 @default.
• W1987418111 cites W1972285889 @default.
• W1987418111 cites W1972556457 @default.
• W1987418111 cites W1977967488 @default.
• W1987418111 cites W1978200825 @default.
• W1987418111 cites W1985178609 @default.
• W1987418111 cites W1987073444 @default.
• W1987418111 cites W2004913761 @default.
• W1987418111 cites W2023232052 @default.
• W1987418111 cites W2026852305 @default.
• W1987418111 cites W2037803546 @default.
• W1987418111 cites W2045050792 @default.
• W1987418111 cites W2047174634 @default.
• W1987418111 cites W2057659396 @default.
• W1987418111 cites W2058075084 @default.
• W1987418111 cites W2064494048 @default.
• W1987418111 cites W2065434575 @default.
• W1987418111 cites W2101292724 @default.
• W1987418111 cites W2103842828 @default.
• W1987418111 cites W2106889107 @default.
• W1987418111 cites W2107852092 @default.
• W1987418111 cites W2112984195 @default.
• W1987418111 cites W2117471635 @default.
• W1987418111 cites W2118266965 @default.
• W1987418111 cites W2123548738 @default.
• W1987418111 cites W2130641564 @default.
• W1987418111 cites W2136483215 @default.
• W1987418111 cites W2138081694 @default.
• W1987418111 cites W2139833746 @default.
• W1987418111 cites W2148979040 @default.
• W1987418111 cites W2150965136 @default.
• W1987418111 cites W2153085895 @default.
• W1987418111 cites W2156841658 @default.
• W1987418111 cites W2161132498 @default.
• W1987418111 cites W2161139786 @default.
• W1987418111 cites W2163629400 @default.
• W1987418111 cites W2167443890 @default.
• W1987418111 cites W2168055071 @default.
• W1987418111 cites W2169621668 @default.
• W1987418111 cites W2795217671 @default.
• W1987418111 cites W76030379 @default.
• W1987418111 doi "https://doi.org/10.1128/aem.06505-11" @default.
• W1987418111 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3298149" @default.
• W1987418111 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22267677" @default.
• W1987418111 hasPublicationYear "2012" @default.
• W1987418111 type Work @default.
• W1987418111 sameAs 1987418111 @default.
• W1987418111 citedByCount "9" @default.
• W1987418111 countsByYear W19874181112013 @default.
• W1987418111 countsByYear W19874181112014 @default.
• W1987418111 countsByYear W19874181112017 @default.
• W1987418111 countsByYear W19874181112019 @default.
• W1987418111 countsByYear W19874181112020 @default.
• W1987418111 crossrefType "journal-article" @default.
• W1987418111 hasAuthorship W1987418111A5002845881 @default.
• W1987418111 hasAuthorship W1987418111A5024504306 @default.
• W1987418111 hasBestOaLocation W19874181111 @default.
• W1987418111 hasConcept C104317684 @default.
• W1987418111 hasConcept C150194340 @default.
• W1987418111 hasConcept C153911025 @default.
• W1987418111 hasConcept C173758957 @default.
• W1987418111 hasConcept C2776273252 @default.
• W1987418111 hasConcept C2777272437 @default.
• W1987418111 hasConcept C2777367657 @default.
• W1987418111 hasConcept C2777932965 @default.
• W1987418111 hasConcept C2779035793 @default.
• W1987418111 hasConcept C2780778831 @default.
• W1987418111 hasConcept C523546767 @default.
• W1987418111 hasConcept C54355233 @default.
• W1987418111 hasConcept C59822182 @default.
• W1987418111 hasConcept C86803240 @default.
• W1987418111 hasConcept C89423630 @default.
• W1987418111 hasConceptScore W1987418111C104317684 @default.
• W1987418111 hasConceptScore W1987418111C150194340 @default.
• W1987418111 hasConceptScore W1987418111C153911025 @default.
• W1987418111 hasConceptScore W1987418111C173758957 @default.
• W1987418111 hasConceptScore W1987418111C2776273252 @default.
• W1987418111 hasConceptScore W1987418111C2777272437 @default.
• W1987418111 hasConceptScore W1987418111C2777367657 @default.
• W1987418111 hasConceptScore W1987418111C2777932965 @default.
• W1987418111 hasConceptScore W1987418111C2779035793 @default.
• W1987418111 hasConceptScore W1987418111C2780778831 @default.
• W1987418111 hasConceptScore W1987418111C523546767 @default.
• W1987418111 hasConceptScore W1987418111C54355233 @default.

• next