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- W1987432876 abstract "The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m = 29.15 mg PCI/mL of adsorbent and K d = 0.0145 mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 = 0.0067 mL/mg s." @default.
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- W1987432876 date "2014-10-10" @default.
- W1987432876 modified "2023-09-23" @default.
- W1987432876 title "Batch Equilibrium and Kinetic Studies of Plasmid pCI Adsorption onto Perfusion Particles" @default.
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- W1987432876 doi "https://doi.org/10.1080/10826076.2014.896818" @default.
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