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- W1987443022 endingPage "9449" @default.
- W1987443022 startingPage "9438" @default.
- W1987443022 abstract "Tryptophan and tyrosine can form radical intermediates that enable long-range, multistep electron transfer (ET) reactions in proteins. This report describes the mechanisms of formation and quenching of a neutral tryptophan radical in azurin, a blue-copper protein that contains native tyrosine (Y108 and Y72) and tryptophan (W48) residues. A long-lived neutral tryptophan radical W48• is formed upon UV-photoexcitation of a zinc(II)-substituted azurin mutant in the presence of an external electron acceptor. The quantum yield of W48• formation (Φ) depends upon the tyrosine residues in the protein. A tyrosine-deficient mutant, Zn(II)Az48W, exhibited a value of Φ = 0.080 with a Co(III) electron acceptor. A nearly identical quantum yield was observed when the electron acceptor was the analogous tyrosine-free, copper(II) mutant; this result for the Zn(II)Az48W:Cu(II)Az48W mixture suggests there is an interprotein ET path. A single tyrosine residue at one of the native positions reduced the quantum yield to 0.062 (Y108) or 0.067 (Y72). Wild-type azurin with two tyrosine residues exhibited a quantum yield of Φ = 0.045. These data indicate that tyrosine is able to quench the tryptophan radical in azurin." @default.
- W1987443022 created "2016-06-24" @default.
- W1987443022 creator A5004858216 @default.
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- W1987443022 creator A5059072077 @default.
- W1987443022 creator A5067396159 @default.
- W1987443022 creator A5090646410 @default.
- W1987443022 date "2015-02-17" @default.
- W1987443022 modified "2023-09-27" @default.
- W1987443022 title "Photogeneration and Quenching of Tryptophan Radical in Azurin" @default.
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- W1987443022 doi "https://doi.org/10.1021/jp511523z" @default.
- W1987443022 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5092234" @default.
- W1987443022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25625660" @default.
- W1987443022 hasPublicationYear "2015" @default.
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