Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987457789> ?p ?o ?g. }
- W1987457789 abstract "ADF/cofilin is a key regulator for actin dynamics during cytokinesis. Its activity is suppressed by phosphorylation and reactivated by dephosphorylation. Little is known, however, about regulatory mechanisms of ADF/cofilin function during formation of contractile ring actin filaments. Using Xenopus cycling extracts, we found that ADF/cofilin was dephosphorylated at prophase and telophase. In addition, constitutively active Rho GTPase induced dephosphorylation of ADF/cofilin in the egg extracts. This dephosphorylation was inhibited by Na(3)VO (4) but not by other conventional phosphatase-inhibitors. We cloned a Xenopus homologue of Slingshot phosphatase (XSSH), originally identified in Drosophila and human as an ADF/cofilin phosphatase, and raised antibody specific for the catalytic domain of XSSH. This inhibitory antibody significantly suppressed the Rho-induced dephosphorylation of ADF/cofilin in extracts, suggesting that the dephosphorylation at telophase is dependent on XSSH. XSSH bound to actin filaments with a dissociation constant of 0.4 microM, and the ADF/cofilin phosphatase activity was increased in the presence of F-actin. When latrunculin A, a G-actin-sequestering drug, was added to extracts, both Rho-induced actin polymerization and dephosphorylation of ADF/cofilin were markedly inhibited. Jasplakinolide, an actin-stabilizing drug, alone induced actin polymerization in the extracts and lead to dephosphorylation of ADF/cofilin. These results suggest that Rho-induced dephosphorylation of ADF/cofilin is dependent on the XSSH activation that is caused by increase in the amount of F-actin induced by Rho signaling. XSSH colocalized with both actin filaments and ADF/cofilin in the actin patches formed on the surface of the early cleavage furrow. Injection of inhibitory antibody blocked cleavage of blastomeres. Thus, XSSH may reorganize actin filaments through dephosphorylation and reactivation of ADF/cofilin at early stage of contractile ring formation." @default.
- W1987457789 created "2016-06-24" @default.
- W1987457789 creator A5011278116 @default.
- W1987457789 creator A5025625340 @default.
- W1987457789 creator A5045285261 @default.
- W1987457789 date "2005-09-01" @default.
- W1987457789 modified "2023-09-25" @default.
- W1987457789 title "Involvement of Slingshot in the Rho-mediated Dephosphorylation of ADF/Cofilin during Xenopus Cleavage" @default.
- W1987457789 cites W114522406 @default.
- W1987457789 cites W1484094963 @default.
- W1987457789 cites W1490646520 @default.
- W1987457789 cites W1538692246 @default.
- W1987457789 cites W1640813462 @default.
- W1987457789 cites W1963892030 @default.
- W1987457789 cites W1971589188 @default.
- W1987457789 cites W1973063454 @default.
- W1987457789 cites W1975912549 @default.
- W1987457789 cites W1978525637 @default.
- W1987457789 cites W1988870503 @default.
- W1987457789 cites W1989478104 @default.
- W1987457789 cites W1991101546 @default.
- W1987457789 cites W1991878174 @default.
- W1987457789 cites W1995757732 @default.
- W1987457789 cites W1998001493 @default.
- W1987457789 cites W1998682793 @default.
- W1987457789 cites W2001289321 @default.
- W1987457789 cites W2002255871 @default.
- W1987457789 cites W2003529245 @default.
- W1987457789 cites W2003736664 @default.
- W1987457789 cites W2005686880 @default.
- W1987457789 cites W2009277812 @default.
- W1987457789 cites W2011107835 @default.
- W1987457789 cites W2014089759 @default.
- W1987457789 cites W2017481817 @default.
- W1987457789 cites W2018307646 @default.
- W1987457789 cites W2020081859 @default.
- W1987457789 cites W2028497798 @default.
- W1987457789 cites W2033989449 @default.
- W1987457789 cites W2039470997 @default.
- W1987457789 cites W2043133065 @default.
- W1987457789 cites W2050002753 @default.
- W1987457789 cites W2063767869 @default.
- W1987457789 cites W2064879077 @default.
- W1987457789 cites W2067901739 @default.
- W1987457789 cites W2068954129 @default.
- W1987457789 cites W2072706075 @default.
- W1987457789 cites W2076481880 @default.
- W1987457789 cites W2077484900 @default.
- W1987457789 cites W2077593465 @default.
- W1987457789 cites W2082971375 @default.
- W1987457789 cites W2083453910 @default.
- W1987457789 cites W2089920751 @default.
- W1987457789 cites W2090206221 @default.
- W1987457789 cites W2093279875 @default.
- W1987457789 cites W2100837269 @default.
- W1987457789 cites W2101108802 @default.
- W1987457789 cites W2108040056 @default.
- W1987457789 cites W2113395636 @default.
- W1987457789 cites W2114536465 @default.
- W1987457789 cites W2115836236 @default.
- W1987457789 cites W2116052541 @default.
- W1987457789 cites W2117494028 @default.
- W1987457789 cites W2126139729 @default.
- W1987457789 cites W2126496496 @default.
- W1987457789 cites W2126546276 @default.
- W1987457789 cites W2131943106 @default.
- W1987457789 cites W2133472355 @default.
- W1987457789 cites W2143634951 @default.
- W1987457789 cites W2160910785 @default.
- W1987457789 cites W2162411580 @default.
- W1987457789 cites W2167324829 @default.
- W1987457789 cites W2170461815 @default.
- W1987457789 cites W2170963117 @default.
- W1987457789 cites W2172048983 @default.
- W1987457789 cites W2186763804 @default.
- W1987457789 cites W2345279659 @default.
- W1987457789 cites W2394487438 @default.
- W1987457789 cites W935855719 @default.
- W1987457789 cites W3142648693 @default.
- W1987457789 cites W3151735281 @default.
- W1987457789 doi "https://doi.org/10.2108/zsj.22.971" @default.
- W1987457789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16219977" @default.
- W1987457789 hasPublicationYear "2005" @default.
- W1987457789 type Work @default.
- W1987457789 sameAs 1987457789 @default.
- W1987457789 citedByCount "16" @default.
- W1987457789 countsByYear W19874577892012 @default.
- W1987457789 countsByYear W19874577892013 @default.
- W1987457789 countsByYear W19874577892015 @default.
- W1987457789 countsByYear W19874577892018 @default.
- W1987457789 countsByYear W19874577892020 @default.
- W1987457789 crossrefType "journal-article" @default.
- W1987457789 hasAuthorship W1987457789A5011278116 @default.
- W1987457789 hasAuthorship W1987457789A5025625340 @default.
- W1987457789 hasAuthorship W1987457789A5045285261 @default.
- W1987457789 hasConcept C104317684 @default.
- W1987457789 hasConcept C11960822 @default.
- W1987457789 hasConcept C125705527 @default.
- W1987457789 hasConcept C142669718 @default.
- W1987457789 hasConcept C1491633281 @default.