FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987492793> ?p ?o ?g. }

• W1987492793 endingPage "24710" @default.
• W1987492793 startingPage "24710" @default.
• W1987492793 abstract "Surface selective patch binding (SPB) interaction occurring between two protein molecules, bovine serum albumin (BSA) and gelatin B (GB), both having same isoelectric pH (pI ≈ 5) and identical pH-zeta potential profile, was systematically examined. BSA : GB mixing ratio r was varied in the range 0.16–2.00 and ionic strength was varied in the range 0–10 mM, which yielded optimum binding ratio r = 1. The binding profiles produced asymmetric bell-like curves with clearly identifiable pairs of transition pHs: onset of intermolecular interaction, formation of soluble complexes and coalescence of the soluble complexes occurring at pHc1,2, pHφ1,2 and pHm respectively. Since pHm could be approached from either lower or higher side of pI, these profiles yielded pairs of pHc and pHφ values. In fact, we found (pHc2 − pI) > (pHc1 − pI), which clearly indicated that initiation of intermolecular associative interaction was not symmetric about pI (pI = pHm for r ≤ 1, an observation not reported hitherto. Secondly, (pHφ2 − pI) ≈ (pHφ1 − pI) implied that the pH at which soluble complexes formed (pHφ) was always located symmetrically about pHm, irrespective of the binding ratio. Higher binding affinity determined from higher value of pHc2 was confirmed from size measurement results. The change in the turbidity maximum Δτ could be correlated as Δτ ∼ I1/2 implying electrostatic screening of SPB with increase in ionic strength (I). This interaction was modelled using a linear combination of attractive and repulsive electrostatic forces which revealed considerable screening of the interaction potential U, consistent with aforesaid experimental data; ΔU ∼ I1/2. Further, it is concluded that intermolecular binding in protein–polyampholyte systems is qualitatively different from that in protein–polyelectrolyte class." @default.
• W1987492793 created "2016-06-24" @default.
• W1987492793 creator A5018766490 @default.
• W1987492793 creator A5028776102 @default.
• W1987492793 creator A5076831416 @default.
• W1987492793 date "2014-01-01" @default.
• W1987492793 modified "2023-10-18" @default.
• W1987492793 title "Is surface patch binding between proteins symmetric about isoelectric pH?" @default.
• W1987492793 cites W1963858830 @default.
• W1987492793 cites W1974455414 @default.
• W1987492793 cites W1981925074 @default.
• W1987492793 cites W1985352713 @default.
• W1987492793 cites W1987328734 @default.
• W1987492793 cites W2004048780 @default.
• W1987492793 cites W2005229258 @default.
• W1987492793 cites W2009792672 @default.
• W1987492793 cites W2018569753 @default.
• W1987492793 cites W2044031937 @default.
• W1987492793 cites W2046407284 @default.
• W1987492793 cites W2047093137 @default.
• W1987492793 cites W2062549138 @default.
• W1987492793 cites W2063820532 @default.
• W1987492793 cites W2067251168 @default.
• W1987492793 cites W2074941649 @default.
• W1987492793 cites W2077007857 @default.
• W1987492793 cites W2080195487 @default.
• W1987492793 cites W2080753081 @default.
• W1987492793 cites W2082549153 @default.
• W1987492793 cites W2084933967 @default.
• W1987492793 cites W2087089396 @default.
• W1987492793 cites W2087278742 @default.
• W1987492793 cites W2106449850 @default.
• W1987492793 cites W2112980795 @default.
• W1987492793 cites W2120763229 @default.
• W1987492793 cites W2132827210 @default.
• W1987492793 cites W2139064613 @default.
• W1987492793 cites W2142152365 @default.
• W1987492793 cites W2144314861 @default.
• W1987492793 cites W2144893435 @default.
• W1987492793 cites W2160061840 @default.
• W1987492793 cites W2164202887 @default.
• W1987492793 cites W2334636839 @default.
• W1987492793 doi "https://doi.org/10.1039/c4ra02372b" @default.
• W1987492793 hasPublicationYear "2014" @default.
• W1987492793 type Work @default.
• W1987492793 sameAs 1987492793 @default.
• W1987492793 citedByCount "10" @default.
• W1987492793 countsByYear W19874927932016 @default.
• W1987492793 countsByYear W19874927932017 @default.
• W1987492793 countsByYear W19874927932018 @default.
• W1987492793 countsByYear W19874927932019 @default.
• W1987492793 countsByYear W19874927932020 @default.
• W1987492793 crossrefType "journal-article" @default.
• W1987492793 hasAuthorship W1987492793A5018766490 @default.
• W1987492793 hasAuthorship W1987492793A5028776102 @default.
• W1987492793 hasAuthorship W1987492793A5076831416 @default.
• W1987492793 hasConcept C113196181 @default.
• W1987492793 hasConcept C147789679 @default.
• W1987492793 hasConcept C155672457 @default.
• W1987492793 hasConcept C166950319 @default.
• W1987492793 hasConcept C171250308 @default.
• W1987492793 hasConcept C178790620 @default.
• W1987492793 hasConcept C181199279 @default.
• W1987492793 hasConcept C184651966 @default.
• W1987492793 hasConcept C185592680 @default.
• W1987492793 hasConcept C187428577 @default.
• W1987492793 hasConcept C192562407 @default.
• W1987492793 hasConcept C32909587 @default.
• W1987492793 hasConcept C43617362 @default.
• W1987492793 hasConcept C50476208 @default.
• W1987492793 hasConcept C53009064 @default.
• W1987492793 hasConcept C55493867 @default.
• W1987492793 hasConcept C71240020 @default.
• W1987492793 hasConcept C8010536 @default.
• W1987492793 hasConcept C86181022 @default.
• W1987492793 hasConcept C86807702 @default.
• W1987492793 hasConcept C90260826 @default.
• W1987492793 hasConcept C96294017 @default.
• W1987492793 hasConceptScore W1987492793C113196181 @default.
• W1987492793 hasConceptScore W1987492793C147789679 @default.
• W1987492793 hasConceptScore W1987492793C155672457 @default.
• W1987492793 hasConceptScore W1987492793C166950319 @default.
• W1987492793 hasConceptScore W1987492793C171250308 @default.
• W1987492793 hasConceptScore W1987492793C178790620 @default.
• W1987492793 hasConceptScore W1987492793C181199279 @default.
• W1987492793 hasConceptScore W1987492793C184651966 @default.
• W1987492793 hasConceptScore W1987492793C185592680 @default.
• W1987492793 hasConceptScore W1987492793C187428577 @default.
• W1987492793 hasConceptScore W1987492793C192562407 @default.
• W1987492793 hasConceptScore W1987492793C32909587 @default.
• W1987492793 hasConceptScore W1987492793C43617362 @default.
• W1987492793 hasConceptScore W1987492793C50476208 @default.
• W1987492793 hasConceptScore W1987492793C53009064 @default.
• W1987492793 hasConceptScore W1987492793C55493867 @default.
• W1987492793 hasConceptScore W1987492793C71240020 @default.
• W1987492793 hasConceptScore W1987492793C8010536 @default.
• W1987492793 hasConceptScore W1987492793C86181022 @default.
• W1987492793 hasConceptScore W1987492793C86807702 @default.

• next