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• W1987506041 endingPage "16558" @default.
• W1987506041 startingPage "16550" @default.
• W1987506041 abstract "Electroreflectance (ER) spectroscopy was used for the estimation of the orientation of heme in a horse heart cytochrome c molecule immobilized on a self-assembled monolayer of 11-mercaptoundecanoic acid on both polycrystalline gold (Au) and single crystalline Au(111) electrodes. The intensity ratio of the ER signal of p-polarized incident light against s-polarized incident light as a function of the incident angle of the light was analyzed in two ways: a doubly degenerate transition for the Soret absorption band of the heme was assumed in one, and the anisotropy of the two orthogonal transitions was taken into account in the other. The doubly degenerate model failed in the expression of the experimental data, pointing to the existence of the anisotropy. Two orthogonal in-plane linear electric dipoles with different magnitudes were assumed to be responsible for the Soret band absorption in a new analysis procedure. This enabled us to fit the experimental data closely to the simulated data. The result revealed that the heme plane is near-vertical to the electrode surface. The need of clarification of the anisotropic heme transition was invoked." @default.
• W1987506041 created "2016-06-24" @default.
• W1987506041 creator A5014693684 @default.
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• W1987506041 date "2006-08-01" @default.
• W1987506041 modified "2023-10-13" @default.
• W1987506041 title "Estimation of the Orientation of Heme in Cytochrome <i>c</i> Immobilized on a Carboxylate-Terminated Alkanethiol Monolayer on a Au Electrode by the Use of Electroreflectance Spectroscopy with Polarized Light Incidence" @default.
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• W1987506041 doi "https://doi.org/10.1021/jp0629240" @default.
• W1987506041 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16913789" @default.
• W1987506041 hasPublicationYear "2006" @default.
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