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- W1987506875 endingPage "e76803" @default.
- W1987506875 startingPage "e76803" @default.
- W1987506875 abstract "The frequency of invasive fungal infections has rapidly increased in recent years. Current clinical treatments are experiencing decreased potency due to severe host toxicity and the emergence of fungal drug resistance. As such, new targets and their corresponding synthetic pathways need to be explored for drug development purposes. In this context, galactofuranose residues, which are employed in fungal cell wall construction, but are notably absent in animals, represent an appealing target. Herein we present the structural and biochemical characterization of UDP-galactose-4-epimerase from Aspergillus nidulans which produces the precursor UDP-galactopyranose required for galactofuranose synthesis. Examination of the structural model revealed both NAD+ and UDP-glucopyranose were bound within the active site cleft in a near identical fashion to that found in the Human epimerase. Mutational studies on the conserved catalytic motif support a similar mechanism to that established for the Human counterpart is likely operational within the A. nidulans epimerase. While the Km and kcat for the enzyme were determined to be 0.11 mM and 12.8 s-1, respectively, a single point mutation, namely L320C, activated the enzyme towards larger N-acetylated substrates. Docking studies designed to probe active site affinity corroborate the experimentally determined activity profiles and support the kinetic inhibition results." @default.
- W1987506875 created "2016-06-24" @default.
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- W1987506875 date "2013-10-07" @default.
- W1987506875 modified "2023-09-23" @default.
- W1987506875 title "Elucidation of Substrate Specificity in Aspergillus nidulans UDP-Galactose-4-Epimerase" @default.
- W1987506875 cites W152085397 @default.
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- W1987506875 cites W1848490583 @default.
- W1987506875 cites W1964458348 @default.
- W1987506875 cites W1965181237 @default.
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- W1987506875 cites W1972692992 @default.
- W1987506875 cites W1984944078 @default.
- W1987506875 cites W1986883777 @default.
- W1987506875 cites W1998840608 @default.
- W1987506875 cites W2004503571 @default.
- W1987506875 cites W2007735611 @default.
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- W1987506875 doi "https://doi.org/10.1371/journal.pone.0076803" @default.
- W1987506875 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3792076" @default.
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- W1987506875 hasPublicationYear "2013" @default.
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