FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987514868> ?p ?o ?g. }

• W1987514868 endingPage "90" @default.
• W1987514868 startingPage "83" @default.
• W1987514868 abstract "In order to analyze the structure-function of multi-subunit RNA polymerases (RNAPs), it is necessary to make site-directed mutations in key residues. Because Saccharomyces cerevisiae RNAP II is isolated as a 12 subunit enzyme that has not been amenable to in vitro reconstitution, making site-directed mutations in a particular subunit presents technical issues. In this work, we demonstrate a method to generate and purify site-directed mutants in the second largest (Rpb2) RNAP II subunit from yeast, using a tandem affinity purification tag. Mutants are analyzed for growth defects in vivo and for defects in transcriptional elongation in vitro. We show that Rpb2 R512A/C located just C-terminal to fork loop 2 (Rpb2 500-511) has transcriptional defects that are distinct from surrounding fork loop 2 region mutants. Rpb2 E529A/D replacements are faster and E529Q is slower than wild type RNAP II in elongation. E529 appears to form an ion pair with K987, an essential active site residue. Mutations are also analyzed within the active site region indicating key residues for catalysis and the importance of a Rpb2 R983-E1028 ion pair. Rpb2 R983Q and E1028Q are defective in escape from a transcriptional stall." @default.
• W1987514868 created "2016-06-24" @default.
• W1987514868 creator A5001615594 @default.
• W1987514868 creator A5011497814 @default.
• W1987514868 creator A5011809151 @default.
• W1987514868 creator A5024724276 @default.
• W1987514868 creator A5069621070 @default.
• W1987514868 creator A5086553695 @default.
• W1987514868 date "2010-01-01" @default.
• W1987514868 modified "2023-10-16" @default.
• W1987514868 title "Site-directed mutagenesis, purification and assay of Saccharomyces cerevisiae RNA polymerase II" @default.
• W1987514868 cites W1483456869 @default.
• W1987514868 cites W1487290611 @default.
• W1987514868 cites W1575542444 @default.
• W1987514868 cites W1591871906 @default.
• W1987514868 cites W1972597018 @default.
• W1987514868 cites W1984497042 @default.
• W1987514868 cites W1984857099 @default.
• W1987514868 cites W1985421947 @default.
• W1987514868 cites W1994342611 @default.
• W1987514868 cites W2002957811 @default.
• W1987514868 cites W2012449152 @default.
• W1987514868 cites W2029667189 @default.
• W1987514868 cites W2043372853 @default.
• W1987514868 cites W2080138031 @default.
• W1987514868 cites W2083387716 @default.
• W1987514868 cites W2100285058 @default.
• W1987514868 cites W2102856492 @default.
• W1987514868 cites W2115659144 @default.
• W1987514868 cites W2120920712 @default.
• W1987514868 cites W2126417186 @default.
• W1987514868 cites W2132198636 @default.
• W1987514868 cites W2142647781 @default.
• W1987514868 cites W2149676146 @default.
• W1987514868 cites W2160444267 @default.
• W1987514868 cites W2163748720 @default.
• W1987514868 cites W2169333620 @default.
• W1987514868 cites W4255796266 @default.
• W1987514868 doi "https://doi.org/10.1016/j.pep.2009.06.016" @default.
• W1987514868 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2783771" @default.
• W1987514868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19567268" @default.
• W1987514868 hasPublicationYear "2010" @default.
• W1987514868 type Work @default.
• W1987514868 sameAs 1987514868 @default.
• W1987514868 citedByCount "17" @default.
• W1987514868 countsByYear W19875148682012 @default.
• W1987514868 countsByYear W19875148682013 @default.
• W1987514868 countsByYear W19875148682015 @default.
• W1987514868 countsByYear W19875148682017 @default.
• W1987514868 countsByYear W19875148682020 @default.
• W1987514868 crossrefType "journal-article" @default.
• W1987514868 hasAuthorship W1987514868A5001615594 @default.
• W1987514868 hasAuthorship W1987514868A5011497814 @default.
• W1987514868 hasAuthorship W1987514868A5011809151 @default.
• W1987514868 hasAuthorship W1987514868A5024724276 @default.
• W1987514868 hasAuthorship W1987514868A5069621070 @default.
• W1987514868 hasAuthorship W1987514868A5086553695 @default.
• W1987514868 hasBestOaLocation W19875148682 @default.
• W1987514868 hasConcept C103408237 @default.
• W1987514868 hasConcept C104292427 @default.
• W1987514868 hasConcept C104317684 @default.
• W1987514868 hasConcept C143065580 @default.
• W1987514868 hasConcept C16318435 @default.
• W1987514868 hasConcept C179247698 @default.
• W1987514868 hasConcept C181199279 @default.
• W1987514868 hasConcept C2776449523 @default.
• W1987514868 hasConcept C2777576037 @default.
• W1987514868 hasConcept C2779222958 @default.
• W1987514868 hasConcept C54355233 @default.
• W1987514868 hasConcept C552990157 @default.
• W1987514868 hasConcept C55493867 @default.
• W1987514868 hasConcept C67705224 @default.
• W1987514868 hasConcept C71095615 @default.
• W1987514868 hasConcept C82381507 @default.
• W1987514868 hasConcept C86803240 @default.
• W1987514868 hasConceptScore W1987514868C103408237 @default.
• W1987514868 hasConceptScore W1987514868C104292427 @default.
• W1987514868 hasConceptScore W1987514868C104317684 @default.
• W1987514868 hasConceptScore W1987514868C143065580 @default.
• W1987514868 hasConceptScore W1987514868C16318435 @default.
• W1987514868 hasConceptScore W1987514868C179247698 @default.
• W1987514868 hasConceptScore W1987514868C181199279 @default.
• W1987514868 hasConceptScore W1987514868C2776449523 @default.
• W1987514868 hasConceptScore W1987514868C2777576037 @default.
• W1987514868 hasConceptScore W1987514868C2779222958 @default.
• W1987514868 hasConceptScore W1987514868C54355233 @default.
• W1987514868 hasConceptScore W1987514868C552990157 @default.
• W1987514868 hasConceptScore W1987514868C55493867 @default.
• W1987514868 hasConceptScore W1987514868C67705224 @default.
• W1987514868 hasConceptScore W1987514868C71095615 @default.
• W1987514868 hasConceptScore W1987514868C82381507 @default.
• W1987514868 hasConceptScore W1987514868C86803240 @default.
• W1987514868 hasIssue "1" @default.
• W1987514868 hasLocation W19875148681 @default.
• W1987514868 hasLocation W19875148682 @default.
• W1987514868 hasLocation W19875148683 @default.
• W1987514868 hasLocation W19875148684 @default.
• W1987514868 hasOpenAccess W1987514868 @default.

• next