FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987609655> ?p ?o ?g. }

• W1987609655 endingPage "2997" @default.
• W1987609655 startingPage "2988" @default.
• W1987609655 abstract "Zein proteins are a complex mixture of polypetides that belong to the alcohol-soluble storage proteins group (prolamines) in corn. These proteins constitute about 50-60% of the total endosperm protein and are classified in different groups on the basis of differences in their solubility and sequence. Among them, zein proteins are considered the majority group showing a high tendency to aggregate what makes their analysis by any analytical method very difficult. Thus, CZE of these proteins requires the use of very complex BGEs noncompatible with online ESI-MS analysis. The aim of this work was to find a new BGE for the CZE separation of zein protein fully compatible with ESI-MS while providing further light on the complex CZE separation of aggregatable proteins. Thus, it is demonstrated in this work that efficient and reproducible CZE separations of zein proteins can be achieved by using a BGE composed of water, ACN, formic acid and ammonium hydroxide. Besides, it is shown that zein analysis is significantly improved by including the effect of an ammonium gradient during their separation. It is experimentally verified that the ammonium gradient can easily be achieved in CZE by either working with a sample zone with a low concentration of ammonium and a BGE with a high concentration, or conversely, working with a sample zone with high ammonium concentration and a BGE with low concentration of ammonium, giving rise in both cases to a significant improvement in the CZE separation of these proteins. It is demonstrated that this procedure can give rise to efficiency improvements of up to 20-fold in the CZE separation of zein proteins. Under optimized conditions, 20 proteins could be separated with average efficiencies higher than 400 000 theoretical plates/m. Some possible explanations of this effect are discussed including stacking, protein-capillary wall adsorption, protein solubility and protein-salt interactions." @default.
• W1987609655 created "2016-06-24" @default.
• W1987609655 creator A5017452379 @default.
• W1987609655 creator A5048148334 @default.
• W1987609655 creator A5057342981 @default.
• W1987609655 date "2007-08-27" @default.
• W1987609655 modified "2023-09-30" @default.
• W1987609655 title "Reproducible and efficient separation of aggregatable zein proteins by CZE using a volatile background electrolyte" @default.
• W1987609655 cites W100823833 @default.
• W1987609655 cites W1964636831 @default.
• W1987609655 cites W1967694847 @default.
• W1987609655 cites W1970836850 @default.
• W1987609655 cites W1977140488 @default.
• W1987609655 cites W1977750637 @default.
• W1987609655 cites W1979023239 @default.
• W1987609655 cites W1979305375 @default.
• W1987609655 cites W1980541251 @default.
• W1987609655 cites W2000828845 @default.
• W1987609655 cites W2002871980 @default.
• W1987609655 cites W2009347362 @default.
• W1987609655 cites W2020977833 @default.
• W1987609655 cites W2022109792 @default.
• W1987609655 cites W2022287033 @default.
• W1987609655 cites W2023653072 @default.
• W1987609655 cites W2026477120 @default.
• W1987609655 cites W2028352008 @default.
• W1987609655 cites W2030907570 @default.
• W1987609655 cites W2032402674 @default.
• W1987609655 cites W2041784653 @default.
• W1987609655 cites W2046704123 @default.
• W1987609655 cites W2050396251 @default.
• W1987609655 cites W2050637333 @default.
• W1987609655 cites W2051987035 @default.
• W1987609655 cites W2052273644 @default.
• W1987609655 cites W2059036768 @default.
• W1987609655 cites W2059759555 @default.
• W1987609655 cites W2060382937 @default.
• W1987609655 cites W2067751516 @default.
• W1987609655 cites W2073317144 @default.
• W1987609655 cites W2073870395 @default.
• W1987609655 cites W2076113143 @default.
• W1987609655 cites W2085142019 @default.
• W1987609655 cites W2088438766 @default.
• W1987609655 cites W2091340459 @default.
• W1987609655 cites W2091505094 @default.
• W1987609655 cites W2092144392 @default.
• W1987609655 cites W2110490531 @default.
• W1987609655 cites W2124958154 @default.
• W1987609655 cites W2140289622 @default.
• W1987609655 cites W2182845228 @default.
• W1987609655 cites W2410824620 @default.
• W1987609655 cites W3022305681 @default.
• W1987609655 cites W4231275087 @default.
• W1987609655 cites W4251755117 @default.
• W1987609655 doi "https://doi.org/10.1002/elps.200700132" @default.
• W1987609655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17661316" @default.
• W1987609655 hasPublicationYear "2007" @default.
• W1987609655 type Work @default.
• W1987609655 sameAs 1987609655 @default.
• W1987609655 citedByCount "10" @default.
• W1987609655 countsByYear W19876096552012 @default.
• W1987609655 countsByYear W19876096552015 @default.
• W1987609655 crossrefType "journal-article" @default.
• W1987609655 hasAuthorship W1987609655A5017452379 @default.
• W1987609655 hasAuthorship W1987609655A5048148334 @default.
• W1987609655 hasAuthorship W1987609655A5057342981 @default.
• W1987609655 hasConcept C113442657 @default.
• W1987609655 hasConcept C147789679 @default.
• W1987609655 hasConcept C155574463 @default.
• W1987609655 hasConcept C17525397 @default.
• W1987609655 hasConcept C178790620 @default.
• W1987609655 hasConcept C185592680 @default.
• W1987609655 hasConcept C2777379063 @default.
• W1987609655 hasConcept C2777879272 @default.
• W1987609655 hasConcept C2779870022 @default.
• W1987609655 hasConcept C2780925461 @default.
• W1987609655 hasConcept C43617362 @default.
• W1987609655 hasConcept C55493867 @default.
• W1987609655 hasConcept C68801617 @default.
• W1987609655 hasConceptScore W1987609655C113442657 @default.
• W1987609655 hasConceptScore W1987609655C147789679 @default.
• W1987609655 hasConceptScore W1987609655C155574463 @default.
• W1987609655 hasConceptScore W1987609655C17525397 @default.
• W1987609655 hasConceptScore W1987609655C178790620 @default.
• W1987609655 hasConceptScore W1987609655C185592680 @default.
• W1987609655 hasConceptScore W1987609655C2777379063 @default.
• W1987609655 hasConceptScore W1987609655C2777879272 @default.
• W1987609655 hasConceptScore W1987609655C2779870022 @default.
• W1987609655 hasConceptScore W1987609655C2780925461 @default.
• W1987609655 hasConceptScore W1987609655C43617362 @default.
• W1987609655 hasConceptScore W1987609655C55493867 @default.
• W1987609655 hasConceptScore W1987609655C68801617 @default.
• W1987609655 hasIssue "17" @default.
• W1987609655 hasLocation W19876096551 @default.
• W1987609655 hasLocation W19876096552 @default.
• W1987609655 hasOpenAccess W1987609655 @default.
• W1987609655 hasPrimaryLocation W19876096551 @default.
• W1987609655 hasRelatedWork W1960555114 @default.

• next