Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987668775> ?p ?o ?g. }
- W1987668775 endingPage "35" @default.
- W1987668775 startingPage "28" @default.
- W1987668775 abstract "The misfolded isoform of the prion protein (PrP(Sc)) possesses many unusual physiochemical properties. Previously, we and others reported on the differential partitioning of PrP(Sc) from plasma derived therapeutic proteins during their purification processes. To understand the driving force behind these partitioning differences, we investigated the effects of various solvent conditions on the precipitation of PrP(Sc). In a physiological buffer, PrP(Sc) remained in the supernatant after low speed centrifugation. At pH 5, PrP(Sc) precipitation was nearly complete regardless of the salt content. PrP(Sc) could also be precipitated at pH 8 by adding ethanol, but this precipitation was salt dependent. Based on these observations, an empirical mathematical model was constructed in which the PrP(Sc) precipitation trends were fully described as a function of solvent pH, salt, and ethanol concentration. This model consistently predicted PrP(Sc) partitioning during cold ethanol precipitation steps used in plasma protein purification processes, as shown by experimentally determined distributions of PrP(Sc) and transmissible spongiform encephalopathy (TSE) infectivity. These results indicate that pH, salt, and ethanol content are the major solvent factors determining the precipitation of the infectious PrP(Sc) in these processes and may provide a useful tool for assessing the differential partitioning of PrP(Sc) in a given solvent environment." @default.
- W1987668775 created "2016-06-24" @default.
- W1987668775 creator A5003040563 @default.
- W1987668775 creator A5008277339 @default.
- W1987668775 creator A5013306589 @default.
- W1987668775 creator A5030012368 @default.
- W1987668775 creator A5030052402 @default.
- W1987668775 creator A5038325380 @default.
- W1987668775 creator A5042678723 @default.
- W1987668775 creator A5073763602 @default.
- W1987668775 creator A5074342032 @default.
- W1987668775 creator A5087386244 @default.
- W1987668775 date "2002-05-01" @default.
- W1987668775 modified "2023-10-16" @default.
- W1987668775 title "Solvent-dependent precipitation of prion protein" @default.
- W1987668775 cites W1662968076 @default.
- W1987668775 cites W1898797172 @default.
- W1987668775 cites W1933863182 @default.
- W1987668775 cites W1967276586 @default.
- W1987668775 cites W1970932468 @default.
- W1987668775 cites W1971916905 @default.
- W1987668775 cites W1973758214 @default.
- W1987668775 cites W1999301383 @default.
- W1987668775 cites W2000427115 @default.
- W1987668775 cites W2003681781 @default.
- W1987668775 cites W2008968500 @default.
- W1987668775 cites W2014742609 @default.
- W1987668775 cites W2021871166 @default.
- W1987668775 cites W2021896470 @default.
- W1987668775 cites W2022392367 @default.
- W1987668775 cites W2022633417 @default.
- W1987668775 cites W2023694825 @default.
- W1987668775 cites W2029440340 @default.
- W1987668775 cites W2029511710 @default.
- W1987668775 cites W2036645353 @default.
- W1987668775 cites W2047411815 @default.
- W1987668775 cites W2057587849 @default.
- W1987668775 cites W2058569537 @default.
- W1987668775 cites W2074255015 @default.
- W1987668775 cites W2078025540 @default.
- W1987668775 cites W2079648943 @default.
- W1987668775 cites W2081303393 @default.
- W1987668775 cites W2083017360 @default.
- W1987668775 cites W2083540358 @default.
- W1987668775 cites W2089604827 @default.
- W1987668775 cites W2090677273 @default.
- W1987668775 cites W2094988704 @default.
- W1987668775 cites W2099576534 @default.
- W1987668775 cites W2109408558 @default.
- W1987668775 cites W2113478642 @default.
- W1987668775 cites W2116063507 @default.
- W1987668775 cites W2320519223 @default.
- W1987668775 cites W2324660492 @default.
- W1987668775 cites W2327387698 @default.
- W1987668775 cites W4240739489 @default.
- W1987668775 cites W4246293002 @default.
- W1987668775 doi "https://doi.org/10.1016/s0167-4838(02)00282-0" @default.
- W1987668775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12009399" @default.
- W1987668775 hasPublicationYear "2002" @default.
- W1987668775 type Work @default.
- W1987668775 sameAs 1987668775 @default.
- W1987668775 citedByCount "31" @default.
- W1987668775 countsByYear W19876687752012 @default.
- W1987668775 countsByYear W19876687752014 @default.
- W1987668775 countsByYear W19876687752015 @default.
- W1987668775 countsByYear W19876687752018 @default.
- W1987668775 countsByYear W19876687752022 @default.
- W1987668775 crossrefType "journal-article" @default.
- W1987668775 hasAuthorship W1987668775A5003040563 @default.
- W1987668775 hasAuthorship W1987668775A5008277339 @default.
- W1987668775 hasAuthorship W1987668775A5013306589 @default.
- W1987668775 hasAuthorship W1987668775A5030012368 @default.
- W1987668775 hasAuthorship W1987668775A5030052402 @default.
- W1987668775 hasAuthorship W1987668775A5038325380 @default.
- W1987668775 hasAuthorship W1987668775A5042678723 @default.
- W1987668775 hasAuthorship W1987668775A5073763602 @default.
- W1987668775 hasAuthorship W1987668775A5074342032 @default.
- W1987668775 hasAuthorship W1987668775A5087386244 @default.
- W1987668775 hasConcept C104317684 @default.
- W1987668775 hasConcept C107054158 @default.
- W1987668775 hasConcept C121332964 @default.
- W1987668775 hasConcept C12554922 @default.
- W1987668775 hasConcept C132946820 @default.
- W1987668775 hasConcept C142724271 @default.
- W1987668775 hasConcept C153294291 @default.
- W1987668775 hasConcept C178790620 @default.
- W1987668775 hasConcept C185592680 @default.
- W1987668775 hasConcept C2776371256 @default.
- W1987668775 hasConcept C2779134260 @default.
- W1987668775 hasConcept C2780161600 @default.
- W1987668775 hasConcept C2780471494 @default.
- W1987668775 hasConcept C3019804061 @default.
- W1987668775 hasConcept C43617362 @default.
- W1987668775 hasConcept C4725764 @default.
- W1987668775 hasConcept C53345823 @default.
- W1987668775 hasConcept C53941386 @default.
- W1987668775 hasConcept C55493867 @default.
- W1987668775 hasConcept C71924100 @default.