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- W1987679631 abstract "Using quantitative S1 nuclease analysis and recombinants which contain the SV4O early promoter region linked to the rabbit β -globin gene coding sequence, we have studied the effect of deletion, inversion and point mutations, located within the 21 bp repeat region, on initiation of transcription from the early-early and late-early startsites in the absence of T-antigen. Our data establish unequivocally that the six GC-rich repeats present in the 21 bp repeat region are essential elements of both the early-early and late-early promoters and that they are not redundant, since mutations, which affect only the GC-rich repeat most proximal to the TATA box, decrease drastically the activity of the early-early promoter, but increase that of the late-early promoter. On the other hand, the four GC-rich repeats most proximal to the 72 bp repeat are common elements of the two overlapping early-early and late-early promoters. Our results, which confirm that the early-early promoter is stronger than the late-early one, also support our previous suggestion that they are in competition for the transcriptional machinery. The general organization of the SV4O early promoter region is discussed." @default.
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- W1987679631 date "1984-01-01" @default.
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- W1987679631 title "Mutational dissection of the 21 bp repeat region of the SV40 early promoter reveals that it contains overlapping elements of the early-early and late-early promoters" @default.
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- W1987679631 doi "https://doi.org/10.1093/nar/12.2.915" @default.
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