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- W1987685910 abstract "At 2 C, all boron accumulated by excised barley roots (Hordeum vulgare L. cv. Herta) remains in the free space; i.e. active uptake is nil at this temperature. Three component fractions of free space B were apparent: (a) a surface contaminant film of B on blotted roots, (b) water free space B, and (c) B reversibly bound in the cell walls. A stoichiometric release of H+ from the roots in the presence of B indicated that B was bound by borate complexes with polysaccharides in the cell walls. Polysaccharide-borate complexes are much less stable than those of monosaccharides, and the bound B fraction could be readily removed by rinsing the roots in the presence of a monomeric polyol possessing the necessary cis-diol configuration. Cell wall material separated from excised barley roots had a B binding capacity 66% greater than that of intact roots. A 30-minute rinse in distilled H2O or 0.5 mm CaSO4 was required to remove all cell wall-bound B from the roots after a 30-minute uptake period. Thus, although B in the contaminant surface film and the water free space is rinsed from the roots within 10 minutes, a 30-minute rinse is essential if all reversibly accumulated B is to be removed from the free space." @default.
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- W1987685910 date "1976-03-01" @default.
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- W1987685910 title "Boron Uptake by Excised Barley Roots" @default.
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- W1987685910 doi "https://doi.org/10.1104/pp.57.3.353" @default.
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