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- W1987761562 abstract "The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day 1 were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) Nω-nitro-l-arginine-methyl ester (l-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later; and (c) l-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with l-NAME abolished this action of alcohol. Injection of l-NAME followed by saline had no influence on the number of IgA + cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide." @default.
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- W1987761562 date "2007-06-01" @default.
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- W1987761562 title "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat" @default.
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- W1987761562 doi "https://doi.org/10.1016/j.intimp.2007.02.010" @default.
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