Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987788305> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1987788305 endingPage "266a" @default.
- W1987788305 startingPage "266a" @default.
- W1987788305 abstract "Using a confocal fluorescence microscope multiparameter fluorescence detection (MFD) enables us to simultaneously collect all fluorescence information such as intensity, lifetime, anisotropy in several spectral ranges) from picoseconds to seconds. MFD and fluorescence correlation spectroscopy is applied to perform single-molecule FRET studies with an ultimate level of precision in determining separations with FRET of 1% of the Förster radius [J.Phys.Chem.B 110, 6970 (2006), J.Phys.Chem.B 112, 8361 (2008)]. In addition we can unambiguously distinguish between stochastic processes and broadening due to static or dynamic heterogeneity. In this way we measured bends and kinks in dsDNA. The high accuracy allowed us the detection of sequence-dependent DNA bending by 16° [PNAS 105, 18773 (2008)]. Moreover we studied the Mg-dependent structural dynamics of a four-way DNA (Holliday-) junction in order to find out whether the postulated extended square structure accumulates indeed as an intermediate or whether it should be considered more as a very short lived transition state. We found a complex Mg dependent kinetics, which must be described by a four species model with only two distinct FRET and two kinetic levels. The species with the same FRET value differ in their conformational flexibility: one is quasi-static, the other is dynamic. Our FRET data are clearly inconsistent with an accumulation of a single extended square junction structure at very low Mg concentrations. Finally we compare the structure and dynamics of DNA- and RNA four-way junctions. Thereby the structure of RNA four-way junction was characterized by 24 FRET distances, which allowed us to prove the existence of 3 of the 4 possible stacking conformers. These studies show that sm FRET studies are valuable tool to complement the structural and dynamic information obtained by X-ray crystallography or NMR spectroscopy." @default.
- W1987788305 created "2016-06-24" @default.
- W1987788305 creator A5005369347 @default.
- W1987788305 creator A5026655832 @default.
- W1987788305 creator A5065144954 @default.
- W1987788305 creator A5066104403 @default.
- W1987788305 creator A5083705187 @default.
- W1987788305 date "2010-01-01" @default.
- W1987788305 modified "2023-10-18" @default.
- W1987788305 title "Single Molecule FRET Measures Structure and Fast Dynamics of DNA and RNA Four-Way Junctions" @default.
- W1987788305 doi "https://doi.org/10.1016/j.bpj.2009.12.1449" @default.
- W1987788305 hasPublicationYear "2010" @default.
- W1987788305 type Work @default.
- W1987788305 sameAs 1987788305 @default.
- W1987788305 citedByCount "3" @default.
- W1987788305 countsByYear W19877883052014 @default.
- W1987788305 countsByYear W19877883052017 @default.
- W1987788305 crossrefType "journal-article" @default.
- W1987788305 hasAuthorship W1987788305A5005369347 @default.
- W1987788305 hasAuthorship W1987788305A5026655832 @default.
- W1987788305 hasAuthorship W1987788305A5065144954 @default.
- W1987788305 hasAuthorship W1987788305A5066104403 @default.
- W1987788305 hasAuthorship W1987788305A5083705187 @default.
- W1987788305 hasBestOaLocation W19877883051 @default.
- W1987788305 hasConcept C102744134 @default.
- W1987788305 hasConcept C111357860 @default.
- W1987788305 hasConcept C120665830 @default.
- W1987788305 hasConcept C121332964 @default.
- W1987788305 hasConcept C12554922 @default.
- W1987788305 hasConcept C130965112 @default.
- W1987788305 hasConcept C136009344 @default.
- W1987788305 hasConcept C144618238 @default.
- W1987788305 hasConcept C147597530 @default.
- W1987788305 hasConcept C159467904 @default.
- W1987788305 hasConcept C178790620 @default.
- W1987788305 hasConcept C185592680 @default.
- W1987788305 hasConcept C2781455991 @default.
- W1987788305 hasConcept C32909587 @default.
- W1987788305 hasConcept C41999313 @default.
- W1987788305 hasConcept C520434653 @default.
- W1987788305 hasConcept C55005982 @default.
- W1987788305 hasConcept C552990157 @default.
- W1987788305 hasConcept C55493867 @default.
- W1987788305 hasConcept C59593255 @default.
- W1987788305 hasConcept C8010536 @default.
- W1987788305 hasConcept C86803240 @default.
- W1987788305 hasConcept C91881484 @default.
- W1987788305 hasConcept C96305047 @default.
- W1987788305 hasConceptScore W1987788305C102744134 @default.
- W1987788305 hasConceptScore W1987788305C111357860 @default.
- W1987788305 hasConceptScore W1987788305C120665830 @default.
- W1987788305 hasConceptScore W1987788305C121332964 @default.
- W1987788305 hasConceptScore W1987788305C12554922 @default.
- W1987788305 hasConceptScore W1987788305C130965112 @default.
- W1987788305 hasConceptScore W1987788305C136009344 @default.
- W1987788305 hasConceptScore W1987788305C144618238 @default.
- W1987788305 hasConceptScore W1987788305C147597530 @default.
- W1987788305 hasConceptScore W1987788305C159467904 @default.
- W1987788305 hasConceptScore W1987788305C178790620 @default.
- W1987788305 hasConceptScore W1987788305C185592680 @default.
- W1987788305 hasConceptScore W1987788305C2781455991 @default.
- W1987788305 hasConceptScore W1987788305C32909587 @default.
- W1987788305 hasConceptScore W1987788305C41999313 @default.
- W1987788305 hasConceptScore W1987788305C520434653 @default.
- W1987788305 hasConceptScore W1987788305C55005982 @default.
- W1987788305 hasConceptScore W1987788305C552990157 @default.
- W1987788305 hasConceptScore W1987788305C55493867 @default.
- W1987788305 hasConceptScore W1987788305C59593255 @default.
- W1987788305 hasConceptScore W1987788305C8010536 @default.
- W1987788305 hasConceptScore W1987788305C86803240 @default.
- W1987788305 hasConceptScore W1987788305C91881484 @default.
- W1987788305 hasConceptScore W1987788305C96305047 @default.
- W1987788305 hasIssue "3" @default.
- W1987788305 hasLocation W19877883051 @default.
- W1987788305 hasOpenAccess W1987788305 @default.
- W1987788305 hasPrimaryLocation W19877883051 @default.
- W1987788305 hasRelatedWork W1577501403 @default.
- W1987788305 hasRelatedWork W1987788305 @default.
- W1987788305 hasRelatedWork W1991154696 @default.
- W1987788305 hasRelatedWork W2007063699 @default.
- W1987788305 hasRelatedWork W2040384706 @default.
- W1987788305 hasRelatedWork W2078308114 @default.
- W1987788305 hasRelatedWork W2120687754 @default.
- W1987788305 hasRelatedWork W2162053394 @default.
- W1987788305 hasRelatedWork W2164771212 @default.
- W1987788305 hasRelatedWork W2417186757 @default.
- W1987788305 hasVolume "98" @default.
- W1987788305 isParatext "false" @default.
- W1987788305 isRetracted "false" @default.
- W1987788305 magId "1987788305" @default.
- W1987788305 workType "article" @default.