FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987788467> ?p ?o ?g. }

• W1987788467 endingPage "37" @default.
• W1987788467 startingPage "32" @default.
• W1987788467 abstract "Two presumptive terpene synthases of unknown biochemical function encoded by the sscg_02150 and sscg_03688 genes of Streptomyces clavuligerus ATCC 27074 were individually expressed in Escherichia coli as N-terminal-His₆-tag proteins, using codon-optimized synthetic genes. Incubation of recombinant SSCG_02150 with farnesyl diphosphate (1, FPP) gave (-)-δ-cadinene (2) while recombinant SSCG_03688 converted FPP to (+)-T-muurolol (3). Individual incubations of (-)-δ-cadinene synthase with [1,1-²H₂]FPP (1a), (1S)-[1-²H]-FPP (1b), and (1R)-[1-²H]-FPP (1c) and NMR analysis of the resulting samples of deuterated (-)-δ-cadinene supported a cyclization mechanism involving the intermediacy of nerolidyl diphosphate (4) leading to a helminthogermacradienyl cation 5. Following a 1,3-hydride shift of the original H-1(si) of FPP, cyclization and deprotonation will give (-)-δ-cadinene. Similar incubations with recombinant SSCG_03688 supported an analogous mechanism for the formation of (+)-T-muurolol (3), also involving a 1,3-hydride shift of the original H-1(si) of FPP." @default.
• W1987788467 created "2016-06-24" @default.
• W1987788467 creator A5026569928 @default.
• W1987788467 creator A5031991326 @default.
• W1987788467 creator A5058917909 @default.
• W1987788467 creator A5089983022 @default.
• W1987788467 date "2011-01-01" @default.
• W1987788467 modified "2023-10-02" @default.
• W1987788467 title "Genome Mining in Streptomyces clavuligerus: Expression and Biochemical Characterization of Two New Cryptic Sesquiterpene Synthases" @default.
• W1987788467 cites W1971234795 @default.
• W1987788467 cites W1974321430 @default.
• W1987788467 cites W1975081365 @default.
• W1987788467 cites W1989740835 @default.
• W1987788467 cites W1990373492 @default.
• W1987788467 cites W1992914002 @default.
• W1987788467 cites W2001921665 @default.
• W1987788467 cites W2008036765 @default.
• W1987788467 cites W2025767103 @default.
• W1987788467 cites W2027583333 @default.
• W1987788467 cites W2027711596 @default.
• W1987788467 cites W2037026236 @default.
• W1987788467 cites W2056400844 @default.
• W1987788467 cites W2062068271 @default.
• W1987788467 cites W2074011778 @default.
• W1987788467 cites W2080950458 @default.
• W1987788467 cites W2089504936 @default.
• W1987788467 cites W2093336502 @default.
• W1987788467 cites W2141367747 @default.
• W1987788467 cites W2147820287 @default.
• W1987788467 cites W2148534902 @default.
• W1987788467 cites W2153242271 @default.
• W1987788467 cites W2165294268 @default.
• W1987788467 cites W2952210622 @default.
• W1987788467 cites W4231644740 @default.
• W1987788467 doi "https://doi.org/10.1016/j.chembiol.2010.11.008" @default.
• W1987788467 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3034312" @default.
• W1987788467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21276937" @default.
• W1987788467 hasPublicationYear "2011" @default.
• W1987788467 type Work @default.
• W1987788467 sameAs 1987788467 @default.
• W1987788467 citedByCount "67" @default.
• W1987788467 countsByYear W19877884672012 @default.
• W1987788467 countsByYear W19877884672013 @default.
• W1987788467 countsByYear W19877884672014 @default.
• W1987788467 countsByYear W19877884672015 @default.
• W1987788467 countsByYear W19877884672016 @default.
• W1987788467 countsByYear W19877884672017 @default.
• W1987788467 countsByYear W19877884672018 @default.
• W1987788467 countsByYear W19877884672019 @default.
• W1987788467 countsByYear W19877884672020 @default.
• W1987788467 countsByYear W19877884672021 @default.
• W1987788467 countsByYear W19877884672022 @default.
• W1987788467 countsByYear W19877884672023 @default.
• W1987788467 crossrefType "journal-article" @default.
• W1987788467 hasAuthorship W1987788467A5026569928 @default.
• W1987788467 hasAuthorship W1987788467A5031991326 @default.
• W1987788467 hasAuthorship W1987788467A5058917909 @default.
• W1987788467 hasAuthorship W1987788467A5089983022 @default.
• W1987788467 hasBestOaLocation W19877884672 @default.
• W1987788467 hasConcept C104317684 @default.
• W1987788467 hasConcept C112243037 @default.
• W1987788467 hasConcept C185592680 @default.
• W1987788467 hasConcept C2776060363 @default.
• W1987788467 hasConcept C2776085463 @default.
• W1987788467 hasConcept C2776211264 @default.
• W1987788467 hasConcept C2777211131 @default.
• W1987788467 hasConcept C2777915650 @default.
• W1987788467 hasConcept C2779920670 @default.
• W1987788467 hasConcept C40767141 @default.
• W1987788467 hasConcept C523546767 @default.
• W1987788467 hasConcept C54355233 @default.
• W1987788467 hasConcept C547475151 @default.
• W1987788467 hasConcept C553450214 @default.
• W1987788467 hasConcept C55493867 @default.
• W1987788467 hasConcept C71240020 @default.
• W1987788467 hasConcept C86803240 @default.
• W1987788467 hasConceptScore W1987788467C104317684 @default.
• W1987788467 hasConceptScore W1987788467C112243037 @default.
• W1987788467 hasConceptScore W1987788467C185592680 @default.
• W1987788467 hasConceptScore W1987788467C2776060363 @default.
• W1987788467 hasConceptScore W1987788467C2776085463 @default.
• W1987788467 hasConceptScore W1987788467C2776211264 @default.
• W1987788467 hasConceptScore W1987788467C2777211131 @default.
• W1987788467 hasConceptScore W1987788467C2777915650 @default.
• W1987788467 hasConceptScore W1987788467C2779920670 @default.
• W1987788467 hasConceptScore W1987788467C40767141 @default.
• W1987788467 hasConceptScore W1987788467C523546767 @default.
• W1987788467 hasConceptScore W1987788467C54355233 @default.
• W1987788467 hasConceptScore W1987788467C547475151 @default.
• W1987788467 hasConceptScore W1987788467C553450214 @default.
• W1987788467 hasConceptScore W1987788467C55493867 @default.
• W1987788467 hasConceptScore W1987788467C71240020 @default.
• W1987788467 hasConceptScore W1987788467C86803240 @default.
• W1987788467 hasIssue "1" @default.
• W1987788467 hasLocation W19877884671 @default.
• W1987788467 hasLocation W19877884672 @default.
• W1987788467 hasLocation W19877884673 @default.
• W1987788467 hasLocation W19877884674 @default.

• next