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- W1987805847 abstract "Four lines of Metaseiulus occidentalis (Nesbitt) were transformed by maternal microinjection. We evaluated the stability of these lines, whether the plasmid DNA was integrated into the nuclear genome, and conducted tests of mode of inheritance to determine whether the transgene was transmitted by males. The putatively transformed lines were identified by standard polymerase chain reaction (PCR) in the G1 generation. After ≈30 generations, the lines were examined by Southern blot hybridization, and the plasmid probe was hybridized to uncut high-molecular-weight DNA from all 4 lines, indicating that the transgene was associated with high-molecular-weight DNA. In restriction-digested Southern blots, 1 of the 4 lines (18) displayed a hybridization pattern consistent with integration of 2 plasmids into the chromosomal DNA. All 4 colonies remained positive by PCR after 150 generations. All 4 colonies examined by RNA-PCR for messenger RNA (mRNA) expression after 100 generations displayed PCR products consistent with transcription of the introduced genetic sequences. In tests of mode of inheritance, plasmid sequences were transmitted by line 18 males, which is consistent with the hypothesis that the plasmid DNA is integrated into the nuclear genome in this line." @default.
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- W1987805847 date "1997-03-01" @default.
- W1987805847 modified "2023-09-28" @default.
- W1987805847 title "Genetic Analysis of Four Lines of Metaseiulus occidentalis (Acari: Phytoseiidae) Transformed by Maternal Microinjection" @default.
- W1987805847 doi "https://doi.org/10.1093/aesa/90.2.237" @default.
- W1987805847 hasPublicationYear "1997" @default.
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