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- W1987862373 abstract "Sheep immunoglobulins were purified and rabbit antisera specific for μ and γ heavy chains and light chains prepared. Lymphocytes obtained by cannulation of the efferent duct of the sheep popliteal node were labeled in vitro with 125I-anti-Ig antibodies. The percentage of lymphocytes labeling for μ, γ or light chains, was, in each case, approximately 20 – 25 %, but cells were most heavily-labeled by anti-μ chain and least by anti-γ chain antibodies. As the sum of the proportions of cells labeling for μ and γ chains (total, 40.6 %) greatly exceeded that labeling for light chains (20.4 %), it was concluded that labeled cells had both γ and μ chains exposed on their surfaces, probably as IgG and IgM. Lymphocytes were titrated against anti-μ or anti-γ chain sera and means (± 1 standard deviation) of 19 200 ± 8200 molecules IgG and 14 700 ± 9300 molecules IgM (19 S, or 73 600 ± 46 800 molecules 8 S) were calculated to be exposed on the surface of each cell labeled by anti-Ig antibodies. It is suggested that those cells from the sheep which bound anti-Ig antibodies were bone marrow-derived lymphocytes as has been found in the mouse." @default.
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- W1987862373 date "1973-01-01" @default.
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- W1987862373 title "Immunoglobulins on the surface of sheep lymphocytes I. Class and cellular distribution" @default.
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- W1987862373 doi "https://doi.org/10.1002/eji.1830030109" @default.
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