Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987912912> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1987912912 endingPage "758" @default.
- W1987912912 startingPage "756" @default.
- W1987912912 abstract "Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lambda light immunoglobulin chains and CD3 antigen by various peroxidase linked detection systems. After reaction with hydrogen peroxide and DAB the sections were immersed in methenamine silver solution at 60 degrees C for three to seven minutes. The light brown stain on the cell membranes of the mantle zone lymphocytes became dark brown and the stronger stain of the plasma cells became black. Mantle zone B lymphocytes and CD3 positive T lymphocytes were precisely outlined even at low magnification and the lymphomas were easily classified as monoclonal or polyclonal. At high magnification, staining was clearer than with the immunogold-silver stain. Cryostat and paraffin wax sections of other tissues immunostained for various antigens showed similar intensification. Silver methenamine provides an easy means of increasing the sensitivity and visual impact of an immunoperoxidase/DAB reaction in any preparation." @default.
- W1987912912 created "2016-06-24" @default.
- W1987912912 creator A5041314720 @default.
- W1987912912 creator A5067580750 @default.
- W1987912912 creator A5081181425 @default.
- W1987912912 date "1991-09-01" @default.
- W1987912912 modified "2023-09-27" @default.
- W1987912912 title "Silver enhancement of polymerised diaminobenzidine: increased sensitivity for immunoperoxidase staining." @default.
- W1987912912 cites W1252201420 @default.
- W1987912912 cites W2011549496 @default.
- W1987912912 cites W2024449985 @default.
- W1987912912 cites W2032552530 @default.
- W1987912912 cites W2064796552 @default.
- W1987912912 cites W2071230078 @default.
- W1987912912 cites W2077914115 @default.
- W1987912912 cites W2096428903 @default.
- W1987912912 cites W2154883832 @default.
- W1987912912 cites W2160131677 @default.
- W1987912912 cites W2326482175 @default.
- W1987912912 cites W4298902812 @default.
- W1987912912 doi "https://doi.org/10.1136/jcp.44.9.756" @default.
- W1987912912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/496724" @default.
- W1987912912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1918404" @default.
- W1987912912 hasPublicationYear "1991" @default.
- W1987912912 type Work @default.
- W1987912912 sameAs 1987912912 @default.
- W1987912912 citedByCount "21" @default.
- W1987912912 countsByYear W19879129122022 @default.
- W1987912912 crossrefType "journal-article" @default.
- W1987912912 hasAuthorship W1987912912A5041314720 @default.
- W1987912912 hasAuthorship W1987912912A5067580750 @default.
- W1987912912 hasAuthorship W1987912912A5081181425 @default.
- W1987912912 hasBestOaLocation W19879129121 @default.
- W1987912912 hasConcept C128102431 @default.
- W1987912912 hasConcept C142724271 @default.
- W1987912912 hasConcept C147483822 @default.
- W1987912912 hasConcept C153460475 @default.
- W1987912912 hasConcept C153911025 @default.
- W1987912912 hasConcept C159654299 @default.
- W1987912912 hasConcept C185592680 @default.
- W1987912912 hasConcept C203014093 @default.
- W1987912912 hasConcept C204232928 @default.
- W1987912912 hasConcept C25330733 @default.
- W1987912912 hasConcept C2781294515 @default.
- W1987912912 hasConcept C4224716 @default.
- W1987912912 hasConcept C542903549 @default.
- W1987912912 hasConcept C71924100 @default.
- W1987912912 hasConcept C74864618 @default.
- W1987912912 hasConcept C86803240 @default.
- W1987912912 hasConcept C956191 @default.
- W1987912912 hasConceptScore W1987912912C128102431 @default.
- W1987912912 hasConceptScore W1987912912C142724271 @default.
- W1987912912 hasConceptScore W1987912912C147483822 @default.
- W1987912912 hasConceptScore W1987912912C153460475 @default.
- W1987912912 hasConceptScore W1987912912C153911025 @default.
- W1987912912 hasConceptScore W1987912912C159654299 @default.
- W1987912912 hasConceptScore W1987912912C185592680 @default.
- W1987912912 hasConceptScore W1987912912C203014093 @default.
- W1987912912 hasConceptScore W1987912912C204232928 @default.
- W1987912912 hasConceptScore W1987912912C25330733 @default.
- W1987912912 hasConceptScore W1987912912C2781294515 @default.
- W1987912912 hasConceptScore W1987912912C4224716 @default.
- W1987912912 hasConceptScore W1987912912C542903549 @default.
- W1987912912 hasConceptScore W1987912912C71924100 @default.
- W1987912912 hasConceptScore W1987912912C74864618 @default.
- W1987912912 hasConceptScore W1987912912C86803240 @default.
- W1987912912 hasConceptScore W1987912912C956191 @default.
- W1987912912 hasIssue "9" @default.
- W1987912912 hasLocation W19879129121 @default.
- W1987912912 hasLocation W19879129122 @default.
- W1987912912 hasLocation W19879129123 @default.
- W1987912912 hasLocation W19879129124 @default.
- W1987912912 hasOpenAccess W1987912912 @default.
- W1987912912 hasPrimaryLocation W19879129121 @default.
- W1987912912 hasRelatedWork W2010998045 @default.
- W1987912912 hasRelatedWork W2014647781 @default.
- W1987912912 hasRelatedWork W2018370991 @default.
- W1987912912 hasRelatedWork W2052178042 @default.
- W1987912912 hasRelatedWork W2068995789 @default.
- W1987912912 hasRelatedWork W2075846906 @default.
- W1987912912 hasRelatedWork W2416382028 @default.
- W1987912912 hasRelatedWork W4231350927 @default.
- W1987912912 hasRelatedWork W4236541504 @default.
- W1987912912 hasRelatedWork W3151039688 @default.
- W1987912912 hasVolume "44" @default.
- W1987912912 isParatext "false" @default.
- W1987912912 isRetracted "false" @default.
- W1987912912 magId "1987912912" @default.
- W1987912912 workType "article" @default.