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- W1987997172 abstract "The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin as a sole carbon source. The S. cerevisiae strain was constructed that is capable of heterologous expression of secreted K. marxianus inulase and is defective in fructose uptake due to null-mutations of the hexokinase structural genes HXK1 and HXK2. When grown in inulin-containing media, this strain is capable of accumulating at least 10% glucose-free fructose in the culture liquid. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 492–497, 1998." @default.
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- W1987997172 date "1998-11-20" @default.
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- W1987997172 title "Inulase-secreting strain ofSaccharomyces cerevisiae produces fructose" @default.
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- W1987997172 doi "https://doi.org/10.1002/(sici)1097-0290(19981120)60:4<492::aid-bit11>3.0.co;2-k" @default.
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