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- W1987999707 abstract "An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation." @default.
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- W1987999707 date "1991-09-01" @default.
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- W1987999707 title "Quantification of Y chromosome bearing spermatozoa of cattle using in situ hybridization" @default.
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- W1987999707 doi "https://doi.org/10.1002/mrd.1080300106" @default.
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