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- W1988035305 abstract "A simple, reliable method was required for the measurement and study of cutaneous lipogenesis in experimental animals. A slice incubation technique was studied using the following tissues. Ear slices from the guinea pig, rat, Mongolian gerbil and golden hamster. Slices from the sebaceous complexes from the guinea pig (supracaudal), rat (preputial), gerbil (ventral) and hamster (dorsal), guinea pig liver and adipose tissue slices were also included for purposes of comparison. Acetate-1,2-14C was used as precursor in all tissues but comparisons were also made with glucose-U-14C and citrate-1,5-14C in most of the systems studied. The results show that the guinea pig ear slice technique gives reproducible results and is the method of choice. Glucose is considered to be the best precursor to use, citrate was relatively inactive under the conditions studied, while data with acetate require caution in interpretation. The sebaceous complexes were not found to be suitable for quantitative studies but should prove invaluable for investigating other aspects of cutaneous lipogenesis. The implications of these results are discussed and the need for caution in the interpretation of comparative data on tissue slices from different organs (even when incubated under identical conditions) is emphasized." @default.
- W1988035305 created "2016-06-24" @default.
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- W1988035305 date "1970-04-01" @default.
- W1988035305 modified "2023-10-17" @default.
- W1988035305 title "Cutaneous Lipogenesis I. Evaluation Of Model Systems and the Utilization of Acetate, Citrate and Glucose as Compared with other Tissues" @default.
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- W1988035305 doi "https://doi.org/10.1111/1523-1747.ep12258582" @default.
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