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- W1988048004 abstract "Metabolic profiling of urinary organic acids from patients with juvenile-onset (Type 1) diabetes mellitus have revealed significantly elevated levels of 2-hydroxybutyric and 4-deoxythreonic acids. To test the hypothesis that these metabolites, as well as 4-deoxyerythronic acid, are derived from L-threonine, stable isotope-labeled threonine was infused into an insulin-deficient dog and the incorporation of 13C into these metabolites was monitored by gas chromatography/mass spectrometry. Electron ionization was relatively insensitive, but positive chemical ionization with ammonia as the reactant gas gave both protonated molecules and [M + NH4]+ ions, which could be analysed by selected ion monitoring. The isotope-labeled species of 2-hydroxybutyric, 4-deoxyerythronic and 4-deoxythreonic acids were observed, but 13C was not incorporated into other organic acids. Thus, it is proposed that L-threonine is a precursor of these metabolites." @default.
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- W1988048004 date "1986-10-01" @default.
- W1988048004 modified "2023-09-27" @default.
- W1988048004 title "Urinary metabolites ofL-threonine in type 1 diabetes determined by combined gas chromatography/chemical ionization mass spectrometry" @default.
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- W1988048004 doi "https://doi.org/10.1002/bms.1200131004" @default.
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