Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988048346> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1988048346 endingPage "127" @default.
- W1988048346 startingPage "120" @default.
- W1988048346 abstract "Storing saliva for nucleic acid diagnostics is problematic in resource-constrained settings. DNA Genotek's OMNIgene™·DISCOVER kit aims to stabilise microbial DNA at room temperature. We evaluate this for long-term storage, determining DNA quantity/purity and human herpesvirus 8 (HHV-8) load as indicator. Viral loads and DNA degradation were assayed over 14months in HHV-8-negative saliva spiked with cell-associated and cell-free virus and saliva collected fresh frozen and into kits from 10 HIV-positive patients. Viral loads remained constant for 6-9months, yielding high quantities of DNA: subsequent losses were ≤48%. Patient samples, frozen or kit stored, produced pure DNA of comparable concentration. Higher HHV-8 detection in frozen saliva resulted from losses during ethanol precipitation using kits. After 14months, DNA degradation was significant in frozen saliva, but that in kits had integrity similar to fresh samples. Storing frozen saliva is detrimental. This kit is well suited for collection, long-term storage, and assay of viral DNA in resource-constrained settings." @default.
- W1988048346 created "2016-06-24" @default.
- W1988048346 creator A5009089006 @default.
- W1988048346 creator A5014037074 @default.
- W1988048346 creator A5014284546 @default.
- W1988048346 creator A5034709604 @default.
- W1988048346 creator A5076921117 @default.
- W1988048346 date "2015-06-01" @default.
- W1988048346 modified "2023-10-06" @default.
- W1988048346 title "Detecting DNA viruses in oral fluids: evaluation of collection and storage methods" @default.
- W1988048346 cites W1537287047 @default.
- W1988048346 cites W1996633580 @default.
- W1988048346 cites W1999394320 @default.
- W1988048346 cites W2011636737 @default.
- W1988048346 cites W2047802360 @default.
- W1988048346 cites W2059799724 @default.
- W1988048346 cites W2072009054 @default.
- W1988048346 cites W2088240443 @default.
- W1988048346 cites W2113272687 @default.
- W1988048346 cites W2121143543 @default.
- W1988048346 cites W2121918395 @default.
- W1988048346 cites W2141682018 @default.
- W1988048346 cites W2152284193 @default.
- W1988048346 doi "https://doi.org/10.1016/j.diagmicrobio.2015.02.013" @default.
- W1988048346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25801777" @default.
- W1988048346 hasPublicationYear "2015" @default.
- W1988048346 type Work @default.
- W1988048346 sameAs 1988048346 @default.
- W1988048346 citedByCount "15" @default.
- W1988048346 countsByYear W19880483462016 @default.
- W1988048346 countsByYear W19880483462017 @default.
- W1988048346 countsByYear W19880483462019 @default.
- W1988048346 countsByYear W19880483462020 @default.
- W1988048346 countsByYear W19880483462021 @default.
- W1988048346 countsByYear W19880483462022 @default.
- W1988048346 crossrefType "journal-article" @default.
- W1988048346 hasAuthorship W1988048346A5009089006 @default.
- W1988048346 hasAuthorship W1988048346A5014037074 @default.
- W1988048346 hasAuthorship W1988048346A5014284546 @default.
- W1988048346 hasAuthorship W1988048346A5034709604 @default.
- W1988048346 hasAuthorship W1988048346A5076921117 @default.
- W1988048346 hasConcept C142462285 @default.
- W1988048346 hasConcept C159047783 @default.
- W1988048346 hasConcept C185592680 @default.
- W1988048346 hasConcept C24107716 @default.
- W1988048346 hasConcept C2522874641 @default.
- W1988048346 hasConcept C504460877 @default.
- W1988048346 hasConcept C552990157 @default.
- W1988048346 hasConcept C55493867 @default.
- W1988048346 hasConcept C86803240 @default.
- W1988048346 hasConceptScore W1988048346C142462285 @default.
- W1988048346 hasConceptScore W1988048346C159047783 @default.
- W1988048346 hasConceptScore W1988048346C185592680 @default.
- W1988048346 hasConceptScore W1988048346C24107716 @default.
- W1988048346 hasConceptScore W1988048346C2522874641 @default.
- W1988048346 hasConceptScore W1988048346C504460877 @default.
- W1988048346 hasConceptScore W1988048346C552990157 @default.
- W1988048346 hasConceptScore W1988048346C55493867 @default.
- W1988048346 hasConceptScore W1988048346C86803240 @default.
- W1988048346 hasIssue "2" @default.
- W1988048346 hasLocation W19880483461 @default.
- W1988048346 hasLocation W19880483462 @default.
- W1988048346 hasOpenAccess W1988048346 @default.
- W1988048346 hasPrimaryLocation W19880483461 @default.
- W1988048346 hasRelatedWork W1487857142 @default.
- W1988048346 hasRelatedWork W2016082374 @default.
- W1988048346 hasRelatedWork W2032469389 @default.
- W1988048346 hasRelatedWork W2064178708 @default.
- W1988048346 hasRelatedWork W2077563405 @default.
- W1988048346 hasRelatedWork W2127289222 @default.
- W1988048346 hasRelatedWork W2155048514 @default.
- W1988048346 hasRelatedWork W2166925281 @default.
- W1988048346 hasRelatedWork W2355191493 @default.
- W1988048346 hasRelatedWork W2359199150 @default.
- W1988048346 hasVolume "82" @default.
- W1988048346 isParatext "false" @default.
- W1988048346 isRetracted "false" @default.
- W1988048346 magId "1988048346" @default.
- W1988048346 workType "article" @default.