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- W1988074780 abstract "In the presence of Mg2+ vanadate was shown to facilitate ouabain bindin to (Na+ + K+)-ATPase in much the say was as Pj does. Thus the hypothesis that vanadate interacts with the phosphate site of the enzyme seems to be supported by ouabain binding experiments At given ouabain concentrations maximum binding is achieved at μM concentrations of vanadate whereas mM concentrations of Pi are needed. Na+ as well as K+ counteract ouabain binding but some cardiac glycoside binding is still possible at in vivo concentrations of these cations. A minor contamination of the enzyme preparations with vanadate could explain the in vitro binding of ouabain that can be obtained with Mg2+ and in the absence of Pi." @default.
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- W1988074780 date "1979-05-01" @default.
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- W1988074780 title "Facilitation of ouabain binding to (Na+ + K+)-ATPase by vanadate at in vivo concentrations" @default.
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- W1988074780 doi "https://doi.org/10.1016/0005-2744(79)90293-6" @default.
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