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• W1988196923 endingPage "38" @default.
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• W1988196923 abstract "Cultured cerebellar granule neurons develop their characteristic axonal and dendritic morphologies in a series of discrete temporal steps highly similar to those observed in situ, initially extending a single process, followed by the extension of a second process from the opposite pole of the cell, both of which develop into axons to generate a bipolar morphology. A mature morphology is attained following the outgrowth of multiple, short dendrites [Powell et al., 1997: J. Neurobiol. 32:223-236]. To determine the relationship between the localization of the Golgi apparatus, the site of microtubule nucleation (the centrosome), and the sites of initial and secondary axonal extension, the intracellular positioning of the Golgi and centrosome was observed during the differentiation of postnatal mouse granule neurons in vitro. The Golgi was labeled using the fluorescent lipid analogue, C5-DMB-Ceramide, or by indirect immunofluorescence using antibodies against the Golgi resident protein, alpha-mannosidase II. At 1-2 days in vitro (DIV), the Golgi was positioned at the base of the initial process in 99% of unipolar cells observed. By 3 DIV, many cells began the transition to a bipolar morphology by extending a short neurite from the pole of the cell opposite to the initial process. The Golgi was observed at this site of secondary outgrowth in 92% of these transitional cells, suggesting that the Golgi was repositioned from the base of the initial process to the site of secondary neurite outgrowth. As the second process elongated and the cells proceeded to the bipolar stage of development, or at later stages when distinct axonal and somatodendritic domains had been established, the Golgi was not consistently positioned at the base of either axons or dendrites, and was most often found at sites on the plasma membrane from which no processes originated. To determine the location of the centrosome in relation to the Golgi during development, granule neurons were labeled with antibodies against gamma-tubulin and optically sectioned using confocal microscopy. The centrosome was consistently co-localized with the Golgi during all stages of differentiation, and also appeared to be repositioned to the base of the newly emerging axon during the transition from a unipolar to a bipolar morphology. These findings indicate that during the early stages of granule cell axonal outgrowth, the Golgi-centrosome is positioned at the base of the initial axon and is then repositioned to the base of the newly emerging secondary axon. Such an intracellular reorientation of these organelles may be important in maintaining the characteristic developmental pattern of granule neurons by establishing the polarized microtubule network and the directed flow of membranous vesicles required for initial axonal elaboration." @default.
• W1988196923 created "2016-06-24" @default.
• W1988196923 creator A5014516188 @default.
• W1988196923 creator A5054929207 @default.
• W1988196923 date "1998-01-01" @default.
• W1988196923 modified "2023-10-16" @default.
• W1988196923 title "The Golgi apparatus and the centrosome are localized to the sites of newly emerging axons in cerebellar granule neurons in vitro" @default.
• W1988196923 cites W1512392705 @default.
• W1988196923 cites W1543566445 @default.
• W1988196923 cites W1559526379 @default.
• W1988196923 cites W1562796888 @default.
• W1988196923 cites W1580763235 @default.
• W1988196923 cites W1585284682 @default.
• W1988196923 cites W1626886456 @default.
• W1988196923 cites W1767420210 @default.
• W1988196923 cites W1812094318 @default.
• W1988196923 cites W1895213472 @default.
• W1988196923 cites W1968725235 @default.
• W1988196923 cites W1969898516 @default.
• W1988196923 cites W1978864905 @default.
• W1988196923 cites W1985499212 @default.
• W1988196923 cites W1993894835 @default.
• W1988196923 cites W1994030229 @default.
• W1988196923 cites W1997122736 @default.
• W1988196923 cites W1997750064 @default.
• W1988196923 cites W1999296966 @default.
• W1988196923 cites W2002687823 @default.
• W1988196923 cites W2005132906 @default.
• W1988196923 cites W2006376420 @default.
• W1988196923 cites W2007904875 @default.
• W1988196923 cites W2009785604 @default.
• W1988196923 cites W2011556695 @default.
• W1988196923 cites W2012196020 @default.
• W1988196923 cites W2015171213 @default.
• W1988196923 cites W2016266703 @default.
• W1988196923 cites W2024918105 @default.
• W1988196923 cites W2034701194 @default.
• W1988196923 cites W2036174328 @default.
• W1988196923 cites W2041080528 @default.
• W1988196923 cites W2041308781 @default.
• W1988196923 cites W2042169083 @default.
• W1988196923 cites W2042957413 @default.
• W1988196923 cites W2043706080 @default.
• W1988196923 cites W2044776028 @default.
• W1988196923 cites W2047038108 @default.
• W1988196923 cites W2049549946 @default.
• W1988196923 cites W2053695273 @default.
• W1988196923 cites W2054199247 @default.
• W1988196923 cites W2058476273 @default.
• W1988196923 cites W2059428912 @default.
• W1988196923 cites W2064829626 @default.
• W1988196923 cites W2065968328 @default.
• W1988196923 cites W2066578425 @default.
• W1988196923 cites W2070393436 @default.
• W1988196923 cites W2070603983 @default.
• W1988196923 cites W2070968243 @default.
• W1988196923 cites W2071534681 @default.
• W1988196923 cites W2076535162 @default.
• W1988196923 cites W2076659391 @default.
• W1988196923 cites W2085357320 @default.
• W1988196923 cites W2092910281 @default.
• W1988196923 cites W2094104389 @default.
• W1988196923 cites W2094725235 @default.
• W1988196923 cites W2098287644 @default.
• W1988196923 cites W2099949096 @default.
• W1988196923 cites W2102160332 @default.
• W1988196923 cites W2102967325 @default.
• W1988196923 cites W2107100596 @default.
• W1988196923 cites W2120375547 @default.
• W1988196923 cites W2122500392 @default.
• W1988196923 cites W2125178516 @default.
• W1988196923 cites W2130892200 @default.
• W1988196923 cites W2131544701 @default.
• W1988196923 cites W2131832714 @default.
• W1988196923 cites W2133931936 @default.
• W1988196923 cites W2136880905 @default.
• W1988196923 cites W2137815145 @default.
• W1988196923 cites W2138070279 @default.
• W1988196923 cites W2138099666 @default.
• W1988196923 cites W2139364889 @default.
• W1988196923 cites W2144892628 @default.
• W1988196923 cites W2159680923 @default.
• W1988196923 cites W2160685946 @default.
• W1988196923 cites W2175885977 @default.
• W1988196923 cites W2178522090 @default.
• W1988196923 cites W2184457366 @default.
• W1988196923 cites W2189417107 @default.
• W1988196923 cites W4238839969 @default.
• W1988196923 doi "https://doi.org/10.1002/(sici)1097-0169(1998)41:1<18::aid-cm2>3.0.co;2-b" @default.
• W1988196923 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9744296" @default.
• W1988196923 hasPublicationYear "1998" @default.
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