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• W1988206245 startingPage "169" @default.
• W1988206245 abstract "Tn1545, a conjugative transposon originally discovered in Streptococcus pneumoniae, has been transferred from Enterococcus faecalis and Bacillus subtilis to Clostridium acetobutylicum NCIB 8052. Transfer between different strains of C. acetobutylicum has also been observed. Insertion of Tn1545 into the C. acetobutylicum chromosome occurred at multiple sites, as shown by Southern hybridization. Although ermAM (erythromycin-resistance) was the most satisfactory marker for primary selection of transconjugants, all three Tn1545-encoded antibiotic resistance genes (aphA-3, ermAM, and tetM) were apparently expressed in C. acetobutylicum. Our results indicate that Tn1545 is potentially useful for undertaking mutagenesis and mutational cloning in this industrially important organism. Transfer of another conjugative transposon, Tn916, from E. faecalis to C. acetobutylicum NCIB 8052 was also apparently detected. Circumstantial evidence suggests that there may be a hot spot for Tn916 insertion in the C. acetobutylicum NCIB 8052 chromosome." @default.
• W1988206245 created "2016-06-24" @default.
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• W1988206245 date "1989-09-01" @default.
• W1988206245 modified "2023-10-17" @default.
• W1988206245 title "Transfer of Tn1545 and Tn916 to Clostridium acetobutylicum" @default.
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• W1988206245 doi "https://doi.org/10.1016/0147-619x(89)90027-9" @default.
• W1988206245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2560219" @default.
• W1988206245 hasPublicationYear "1989" @default.
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