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- W1988251749 abstract "The uptake of <sup>32</sup>P by viable deposits of <i>Streptococcus sanguis</i> grown on wires in broth was largely dependent upon the presence of an external carbohydrate source. Viable deposits in the absence of carbohydrate, and also deposits in which the cells had been killed, showed relatively little uptake. Meta- bolized <sup>32</sup>P was located mainly in the nucleic acids fraction. <sup>32</sup>P was retained within the viable cells upon extraction of deposits with saline for 96 h at 37 °C and acid saline pH 3.5 (lactic acid) for 72 h at 37 °C. Destruction of matrix with dextranase did not liberate a significant amount of ‘bound’ phosphorus. A possible source of the phosphorus liberated from viable cells in the absence of carbohydrate could be cellular RNA, which has been reported to be utilized by some bacteria during endogenous metabolism. In contrast, <sup>45</sup>Ca showed no selective uptake by deposits and was rapidly removed upon aqueous extraction. The release of both <sup>45</sup>Ca and <sup>32</sup>P from enamel was restricted by surface deposits containing viable and dead cells. The metabolism of phosphorus by viable cells, and the ‘ion barrier’ effects of the deposits which continue after death of the cells, might explain the histological appearance of the <i>in vitro</i> enamel carious lesions described in a previous study." @default.
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- W1988251749 date "1973-01-01" @default.
- W1988251749 modified "2023-10-14" @default.
- W1988251749 title "The Localization and Retention of <sup>32</sup>P and <sup>45</sup>Ca within Surface Deposits of Streptococcus sanguis and the Influence of such Deposits on the Release of these Isotopes from Enamel" @default.
- W1988251749 doi "https://doi.org/10.1159/000259843" @default.
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