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- W1988280690 abstract "7-Ketocholesterol (7KCh) accumulates in oxidized lipoprotein deposits and is known to be involved in macrophage foam cell formation and atherosclerosis. 7-KCh is present in the primate retina and is associated with oxidized lipoprotein deposits located in the choriocapillaris, Bruch's membrane, and retinal pigment epithelium (RPE). 7-KCh can also be formed in the retina as a consequence of light-induced iron release. The purpose of this study was to examine the signaling pathways involved in the 7KCh-mediated inflammatory response focusing on three cytokines, VEGF, IL-6, and IL-8.ARPE-19 cells were treated with 7KCh solubilized in hydroxypropyl-β-cyclodextrin. Cytokines were quantified by qRT-PCR (mRNA) and ELISA (protein) using commercially available products. NFκB activation was determined by IκBα mRNA induction.Treatment of ARPE-19 cells with 15 μM 7KCh markedly induced the expression of VEGF, IL-6, and IL-8. No increase in NOX-4 expression or ROS formation was detected. 7KCh induced the phosphorylation of ERK1/2 and p38MAPK, and inhibitors to these kinases markedly reduced the cytokine expression but did not affect the IκBα mRNA expression. By contrast, inhibition of PI3K and PKCζ significantly decreased the cytokine and IκBα mRNA expression. Inhibition of the IκB kinase complex essentially ablated all cytokine induction.7KCh induces cytokines via three kinase signaling pathways, AKT-PKCζ-NFκB, p38 MAPK, and ERK. The MAPK/ERK pathways seem to preferentially enhance cytokine induction downstream from NFκB activation. The results of this study suggest that 7KCh activates these pathways through interactions in the plasma membrane, but the mechanism(s) remains unknown." @default.
- W1988280690 created "2016-06-24" @default.
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- W1988280690 date "2010-10-01" @default.
- W1988280690 modified "2023-09-27" @default.
- W1988280690 title "7-Ketocholesterol–Induced Inflammation: Involvement of Multiple Kinase Signaling Pathways via NFκB but Independently of Reactive Oxygen Species Formation" @default.
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- W1988280690 cites W1488191987 @default.
- W1988280690 cites W1493322929 @default.
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- W1988280690 cites W1583827678 @default.
- W1988280690 cites W1791013575 @default.
- W1988280690 cites W1822715048 @default.
- W1988280690 cites W1963596901 @default.
- W1988280690 cites W1965528611 @default.
- W1988280690 cites W1965909642 @default.
- W1988280690 cites W1966766618 @default.
- W1988280690 cites W1968647954 @default.
- W1988280690 cites W1970314190 @default.
- W1988280690 cites W1971263149 @default.
- W1988280690 cites W1973026307 @default.
- W1988280690 cites W1981249224 @default.
- W1988280690 cites W1983190703 @default.
- W1988280690 cites W1985578820 @default.
- W1988280690 cites W1990632046 @default.
- W1988280690 cites W1994079695 @default.
- W1988280690 cites W1997798902 @default.
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- W1988280690 cites W2004767898 @default.
- W1988280690 cites W2004965791 @default.
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- W1988280690 cites W2016870402 @default.
- W1988280690 cites W2022991915 @default.
- W1988280690 cites W2023243666 @default.
- W1988280690 cites W2023817080 @default.
- W1988280690 cites W2023832715 @default.
- W1988280690 cites W2025317498 @default.
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- W1988280690 cites W2033060255 @default.
- W1988280690 cites W2033781074 @default.
- W1988280690 cites W2037697286 @default.
- W1988280690 cites W2046335519 @default.
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- W1988280690 cites W2056993355 @default.
- W1988280690 cites W2063519410 @default.
- W1988280690 cites W2063706010 @default.
- W1988280690 cites W2064513293 @default.
- W1988280690 cites W2066503969 @default.
- W1988280690 cites W2068297435 @default.
- W1988280690 cites W2069276202 @default.
- W1988280690 cites W2070291679 @default.
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- W1988280690 cites W2118233199 @default.
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- W1988280690 doi "https://doi.org/10.1167/iovs.09-4854" @default.
- W1988280690 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3066624" @default.
- W1988280690 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20554621" @default.
- W1988280690 hasPublicationYear "2010" @default.
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