Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988366950> ?p ?o ?g. }
- W1988366950 endingPage "191" @default.
- W1988366950 startingPage "185" @default.
- W1988366950 abstract "Acetohydroxy acid synthase (AHAS) is one of several enzymes that require thiamine diphosphate and a divalent cation as essential cofactors. Recently, the three-dimensional structure of the enzyme from yeast has been determined [Pang et al., J. Mol. Biol. 317 (2002) 249-262]. While this structure sheds light on the binding of the cofactors and the reaction mechanism, the interactions between the substrates and the enzyme remain unclear. We have studied the pH dependence of kinetic parameters in order to obtain information about the chemical mechanism in the active site. Data are consistent with a mechanism in which substrate selectively catalyzed to the enzyme with an unprotonated base having a pK of 6.48, and a protonated group having a pK of 8.25 for catalysis. The temperature dependence of kinetic parameters was pH-dependent, and the enthalpies of ionization, DeltaH(ion), calculated from the slope of pK(1) and pK(2) are both pH-independent. The solvent perturbation of kinetic parameters was pH-dependent, and the pK(1) from the acidic side and the pK(2) from the basic side were shifted down 0.4 pH units and shifted up 0.6 units as water was replaced by 15% ethanol, respectively. The data are discussed in terms of the acid-base chemical mechanism." @default.
- W1988366950 created "2016-06-24" @default.
- W1988366950 creator A5010364211 @default.
- W1988366950 creator A5025657554 @default.
- W1988366950 creator A5027745513 @default.
- W1988366950 creator A5046575574 @default.
- W1988366950 creator A5057041507 @default.
- W1988366950 creator A5071342667 @default.
- W1988366950 creator A5089958431 @default.
- W1988366950 date "2003-10-21" @default.
- W1988366950 modified "2023-10-18" @default.
- W1988366950 title "The active site and mechanism of action of recombinant acetohydroxy acid synthase from tobacco" @default.
- W1988366950 cites W1526775414 @default.
- W1988366950 cites W1581959325 @default.
- W1988366950 cites W1627898652 @default.
- W1988366950 cites W1976601089 @default.
- W1988366950 cites W1979292671 @default.
- W1988366950 cites W1983879546 @default.
- W1988366950 cites W1986433763 @default.
- W1988366950 cites W1988847460 @default.
- W1988366950 cites W2007100385 @default.
- W1988366950 cites W2018779619 @default.
- W1988366950 cites W2020202061 @default.
- W1988366950 cites W2038483491 @default.
- W1988366950 cites W2045953953 @default.
- W1988366950 cites W2070128378 @default.
- W1988366950 cites W2074259659 @default.
- W1988366950 cites W2074493971 @default.
- W1988366950 cites W2086160038 @default.
- W1988366950 cites W2098013527 @default.
- W1988366950 cites W2102892942 @default.
- W1988366950 cites W2139451514 @default.
- W1988366950 cites W2155612513 @default.
- W1988366950 cites W2171466907 @default.
- W1988366950 cites W2326706821 @default.
- W1988366950 cites W2969273703 @default.
- W1988366950 doi "https://doi.org/10.1016/s0014-5793(03)01177-3" @default.
- W1988366950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14644413" @default.
- W1988366950 hasPublicationYear "2003" @default.
- W1988366950 type Work @default.
- W1988366950 sameAs 1988366950 @default.
- W1988366950 citedByCount "8" @default.
- W1988366950 countsByYear W19883669502015 @default.
- W1988366950 crossrefType "journal-article" @default.
- W1988366950 hasAuthorship W1988366950A5010364211 @default.
- W1988366950 hasAuthorship W1988366950A5025657554 @default.
- W1988366950 hasAuthorship W1988366950A5027745513 @default.
- W1988366950 hasAuthorship W1988366950A5046575574 @default.
- W1988366950 hasAuthorship W1988366950A5057041507 @default.
- W1988366950 hasAuthorship W1988366950A5071342667 @default.
- W1988366950 hasAuthorship W1988366950A5089958431 @default.
- W1988366950 hasConcept C111368507 @default.
- W1988366950 hasConcept C127313418 @default.
- W1988366950 hasConcept C145148216 @default.
- W1988366950 hasConcept C161790260 @default.
- W1988366950 hasConcept C178790620 @default.
- W1988366950 hasConcept C181199279 @default.
- W1988366950 hasConcept C185592680 @default.
- W1988366950 hasConcept C197957613 @default.
- W1988366950 hasConcept C2777289219 @default.
- W1988366950 hasConcept C30095370 @default.
- W1988366950 hasConcept C41183919 @default.
- W1988366950 hasConcept C55493867 @default.
- W1988366950 hasConcept C71240020 @default.
- W1988366950 hasConcept C74884574 @default.
- W1988366950 hasConceptScore W1988366950C111368507 @default.
- W1988366950 hasConceptScore W1988366950C127313418 @default.
- W1988366950 hasConceptScore W1988366950C145148216 @default.
- W1988366950 hasConceptScore W1988366950C161790260 @default.
- W1988366950 hasConceptScore W1988366950C178790620 @default.
- W1988366950 hasConceptScore W1988366950C181199279 @default.
- W1988366950 hasConceptScore W1988366950C185592680 @default.
- W1988366950 hasConceptScore W1988366950C197957613 @default.
- W1988366950 hasConceptScore W1988366950C2777289219 @default.
- W1988366950 hasConceptScore W1988366950C30095370 @default.
- W1988366950 hasConceptScore W1988366950C41183919 @default.
- W1988366950 hasConceptScore W1988366950C55493867 @default.
- W1988366950 hasConceptScore W1988366950C71240020 @default.
- W1988366950 hasConceptScore W1988366950C74884574 @default.
- W1988366950 hasIssue "2" @default.
- W1988366950 hasLocation W19883669501 @default.
- W1988366950 hasLocation W19883669502 @default.
- W1988366950 hasOpenAccess W1988366950 @default.
- W1988366950 hasPrimaryLocation W19883669501 @default.
- W1988366950 hasRelatedWork W1550634984 @default.
- W1988366950 hasRelatedWork W2000898535 @default.
- W1988366950 hasRelatedWork W2010596396 @default.
- W1988366950 hasRelatedWork W2067812218 @default.
- W1988366950 hasRelatedWork W2168135385 @default.
- W1988366950 hasRelatedWork W2249621633 @default.
- W1988366950 hasRelatedWork W3022531063 @default.
- W1988366950 hasRelatedWork W3095664373 @default.
- W1988366950 hasRelatedWork W4385563169 @default.
- W1988366950 hasRelatedWork W4385563181 @default.
- W1988366950 hasVolume "555" @default.
- W1988366950 isParatext "false" @default.
- W1988366950 isRetracted "false" @default.
- W1988366950 magId "1988366950" @default.