FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988366997> ?p ?o ?g. }

• W1988366997 endingPage "624" @default.
• W1988366997 startingPage "617" @default.
• W1988366997 abstract "The viability of cells within the acidic microenvironment found in solid tumours is expected to depend on the regulation of intracellular pH (pHi). 5-(N,N-hexamethylene) amiloride (HMA) is a potent inhibitor of the Na+/H+ antiport, a major mechanism for the regulation of pHi. We have therefore studied the cytotoxicity of HMA in combination with nigericin, a cell-acidifying agent, for EMT-6 cells in monolayer cell culture, in spheroids and in a murine tumour model. The combination of nigericin and HMA was toxic to cells in tissue culture at extracellular pH (pHe) < or = 6.8 (as may be found in tumours) but not at pH 7.0 or above (as in most normal tissues). Compared with amiloride, the relative potency of HMA in causing in vitro cytotoxicity (approximately 100-fold) was similar to that for inhibition of the Na+/H+ antiport. The fluorescent probe Hoechst 33342 was used with flow cytometry to study the cytotoxicity of HMA and nigericin at different depths in multicellular tumour spheroids. Only small differences in the level of cell survival were observed, but higher concentrations of HMA were required as compared with those giving equal levels of survival in monolayer culture. The pharmacokinetics of HMA in mice was studied by using high-performance liquid chromatography: after intraperitoneal injection of 20 micrograms g-1, the plasma level of HMA peaked at 8 microM after about 15 min and decreased to 1 microM at 120 min; the half-life was 35 min. Nigericin and HMA, at doses of 1.25 micrograms g-1 and 10 micrograms g-1 respectively, failed to cause significant cell killing in the EMT-6 murine tumour, but the surviving fraction was reduced to approximately 0.004 when hydralazine was administered with nigericin and HMA. Local tumour irradiation (15 Gy), followed by treatment with these drugs, led to cell killing that was additive to the effects of drugs and radiation alone, so that hypoxic cells which survived radiation did not appear more sensitive to pH-dependent drug treatment. Acid-mediated therapy can lead to cell death in murine solid tumours, but further measures will be required before the strategy can be exploited clinically." @default.
• W1988366997 created "2016-06-24" @default.
• W1988366997 creator A5038721944 @default.
• W1988366997 creator A5063846121 @default.
• W1988366997 date "1994-10-01" @default.
• W1988366997 modified "2023-10-11" @default.
• W1988366997 title "Inhibition of the regulation of intracellular pH: potential of 5-(N,N-hexamethylene) amiloride in tumour-selective therapy" @default.
• W1988366997 cites W1558696846 @default.
• W1988366997 cites W1575778999 @default.
• W1988366997 cites W1578086030 @default.
• W1988366997 cites W1592146427 @default.
• W1988366997 cites W1658081095 @default.
• W1988366997 cites W1790873293 @default.
• W1988366997 cites W1834876440 @default.
• W1988366997 cites W1839358117 @default.
• W1988366997 cites W1914697351 @default.
• W1988366997 cites W1947203665 @default.
• W1988366997 cites W1954857248 @default.
• W1988366997 cites W1956944003 @default.
• W1988366997 cites W1964986583 @default.
• W1988366997 cites W1976596057 @default.
• W1988366997 cites W1983768617 @default.
• W1988366997 cites W2011421342 @default.
• W1988366997 cites W2013646710 @default.
• W1988366997 cites W2016284736 @default.
• W1988366997 cites W2020042606 @default.
• W1988366997 cites W2022128427 @default.
• W1988366997 cites W2041492743 @default.
• W1988366997 cites W2041585886 @default.
• W1988366997 cites W2043549986 @default.
• W1988366997 cites W2046788495 @default.
• W1988366997 cites W2074061850 @default.
• W1988366997 cites W2081586048 @default.
• W1988366997 cites W2086631379 @default.
• W1988366997 cites W2088687690 @default.
• W1988366997 cites W2106973449 @default.
• W1988366997 cites W2110551469 @default.
• W1988366997 cites W2119434127 @default.
• W1988366997 cites W2122880329 @default.
• W1988366997 cites W2165884308 @default.
• W1988366997 cites W2317309164 @default.
• W1988366997 cites W2318996479 @default.
• W1988366997 cites W2332231957 @default.
• W1988366997 cites W2411135157 @default.
• W1988366997 doi "https://doi.org/10.1038/bjc.1994.360" @default.
• W1988366997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2033413" @default.
• W1988366997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7917906" @default.
• W1988366997 hasPublicationYear "1994" @default.
• W1988366997 type Work @default.
• W1988366997 sameAs 1988366997 @default.
• W1988366997 citedByCount "36" @default.
• W1988366997 countsByYear W19883669972013 @default.
• W1988366997 countsByYear W19883669972014 @default.
• W1988366997 countsByYear W19883669972016 @default.
• W1988366997 countsByYear W19883669972017 @default.
• W1988366997 countsByYear W19883669972018 @default.
• W1988366997 countsByYear W19883669972019 @default.
• W1988366997 countsByYear W19883669972021 @default.
• W1988366997 countsByYear W19883669972022 @default.
• W1988366997 countsByYear W19883669972023 @default.
• W1988366997 crossrefType "journal-article" @default.
• W1988366997 hasAuthorship W1988366997A5038721944 @default.
• W1988366997 hasAuthorship W1988366997A5063846121 @default.
• W1988366997 hasBestOaLocation W19883669971 @default.
• W1988366997 hasConcept C109316439 @default.
• W1988366997 hasConcept C117262875 @default.
• W1988366997 hasConcept C134897140 @default.
• W1988366997 hasConcept C153911025 @default.
• W1988366997 hasConcept C178790620 @default.
• W1988366997 hasConcept C185592680 @default.
• W1988366997 hasConcept C202751555 @default.
• W1988366997 hasConcept C2777330628 @default.
• W1988366997 hasConcept C2779608910 @default.
• W1988366997 hasConcept C28406088 @default.
• W1988366997 hasConcept C41625074 @default.
• W1988366997 hasConcept C537181965 @default.
• W1988366997 hasConcept C54355233 @default.
• W1988366997 hasConcept C553184892 @default.
• W1988366997 hasConcept C55493867 @default.
• W1988366997 hasConcept C57181701 @default.
• W1988366997 hasConcept C79879829 @default.
• W1988366997 hasConcept C81885089 @default.
• W1988366997 hasConcept C86803240 @default.
• W1988366997 hasConcept C98274493 @default.
• W1988366997 hasConceptScore W1988366997C109316439 @default.
• W1988366997 hasConceptScore W1988366997C117262875 @default.
• W1988366997 hasConceptScore W1988366997C134897140 @default.
• W1988366997 hasConceptScore W1988366997C153911025 @default.
• W1988366997 hasConceptScore W1988366997C178790620 @default.
• W1988366997 hasConceptScore W1988366997C185592680 @default.
• W1988366997 hasConceptScore W1988366997C202751555 @default.
• W1988366997 hasConceptScore W1988366997C2777330628 @default.
• W1988366997 hasConceptScore W1988366997C2779608910 @default.
• W1988366997 hasConceptScore W1988366997C28406088 @default.
• W1988366997 hasConceptScore W1988366997C41625074 @default.
• W1988366997 hasConceptScore W1988366997C537181965 @default.
• W1988366997 hasConceptScore W1988366997C54355233 @default.
• W1988366997 hasConceptScore W1988366997C553184892 @default.

• next