Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988394499> ?p ?o ?g. }
- W1988394499 endingPage "1255" @default.
- W1988394499 startingPage "1248" @default.
- W1988394499 abstract "In the daily clinical routine, numerous synthetic medical devices are implanted in the human body, either temporarily or permanently. The synthetic material most often implanted is polydimethylsiloxane (silicone). Numerous studies have demonstrated that silicone is encompassed in a connective tissue capsule by the body, preventing integration into the surrounding tissue. This can result in complications. The aim of our study was to develop a simple procedure to functionalize the silicone surface, thereby positively affecting the material's biocompatibility. By combining a silanization with the use of ester activation, a reactive amino group is generated, which can bind any free carboxyl group. Directional crosslinking of a near-infrared-conjugated fluorophore antibody to the activated silicone surface could be demonstrated on a dose-dependent basis. The redox reaction at a silicone surface coated with an HRP-conjugated antibody caused by the addition of NBT/BCIP could be shown. Covering the silicone discs with an anti-FAS-antibody coating followed by a coincubation with FAS-sensitive T-cells allowed highly significant detection of caspase-3. In summary, our crosslinking procedure enables the stable binding of proteins without the loss of biological function. Through this process, silicones could be endowed with new functions which could improve their biocompatibility." @default.
- W1988394499 created "2016-06-24" @default.
- W1988394499 creator A5005821687 @default.
- W1988394499 creator A5007894310 @default.
- W1988394499 creator A5062434872 @default.
- W1988394499 creator A5069131330 @default.
- W1988394499 creator A5073625454 @default.
- W1988394499 date "2012-02-23" @default.
- W1988394499 modified "2023-10-18" @default.
- W1988394499 title "Covalent vectored binding of functional proteins by bifunctional crosslinking at silicone interfaces" @default.
- W1988394499 cites W1965147914 @default.
- W1988394499 cites W1972508043 @default.
- W1988394499 cites W1976356486 @default.
- W1988394499 cites W1979289623 @default.
- W1988394499 cites W1981906522 @default.
- W1988394499 cites W1991300200 @default.
- W1988394499 cites W1994988324 @default.
- W1988394499 cites W1995994181 @default.
- W1988394499 cites W2001646556 @default.
- W1988394499 cites W2004793681 @default.
- W1988394499 cites W2018977020 @default.
- W1988394499 cites W2022781946 @default.
- W1988394499 cites W2024663839 @default.
- W1988394499 cites W2033935793 @default.
- W1988394499 cites W2036374139 @default.
- W1988394499 cites W2039914303 @default.
- W1988394499 cites W2041571299 @default.
- W1988394499 cites W2044142797 @default.
- W1988394499 cites W2047773026 @default.
- W1988394499 cites W2048366073 @default.
- W1988394499 cites W2048904093 @default.
- W1988394499 cites W2053014600 @default.
- W1988394499 cites W2060212870 @default.
- W1988394499 cites W2066573870 @default.
- W1988394499 cites W2070641387 @default.
- W1988394499 cites W2074537892 @default.
- W1988394499 cites W2076452272 @default.
- W1988394499 cites W2081334854 @default.
- W1988394499 cites W2082027989 @default.
- W1988394499 cites W2082335907 @default.
- W1988394499 cites W2082448223 @default.
- W1988394499 cites W2087330045 @default.
- W1988394499 cites W2103722820 @default.
- W1988394499 cites W2131469468 @default.
- W1988394499 cites W2132958134 @default.
- W1988394499 cites W2135231668 @default.
- W1988394499 cites W2136979131 @default.
- W1988394499 cites W2143685603 @default.
- W1988394499 cites W2144629070 @default.
- W1988394499 cites W2167541320 @default.
- W1988394499 cites W24846033 @default.
- W1988394499 doi "https://doi.org/10.1002/jbm.a.34008" @default.
- W1988394499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22359358" @default.
- W1988394499 hasPublicationYear "2012" @default.
- W1988394499 type Work @default.
- W1988394499 sameAs 1988394499 @default.
- W1988394499 citedByCount "6" @default.
- W1988394499 countsByYear W19883944992013 @default.
- W1988394499 countsByYear W19883944992014 @default.
- W1988394499 countsByYear W19883944992015 @default.
- W1988394499 countsByYear W19883944992016 @default.
- W1988394499 countsByYear W19883944992018 @default.
- W1988394499 countsByYear W19883944992022 @default.
- W1988394499 crossrefType "journal-article" @default.
- W1988394499 hasAuthorship W1988394499A5005821687 @default.
- W1988394499 hasAuthorship W1988394499A5007894310 @default.
- W1988394499 hasAuthorship W1988394499A5062434872 @default.
- W1988394499 hasAuthorship W1988394499A5069131330 @default.
- W1988394499 hasAuthorship W1988394499A5073625454 @default.
- W1988394499 hasConcept C112613896 @default.
- W1988394499 hasConcept C115537861 @default.
- W1988394499 hasConcept C12554922 @default.
- W1988394499 hasConcept C127413603 @default.
- W1988394499 hasConcept C136229726 @default.
- W1988394499 hasConcept C159985019 @default.
- W1988394499 hasConcept C171250308 @default.
- W1988394499 hasConcept C178790620 @default.
- W1988394499 hasConcept C180577832 @default.
- W1988394499 hasConcept C185592680 @default.
- W1988394499 hasConcept C188027245 @default.
- W1988394499 hasConcept C191897082 @default.
- W1988394499 hasConcept C192562407 @default.
- W1988394499 hasConcept C2777230088 @default.
- W1988394499 hasConcept C2779769944 @default.
- W1988394499 hasConcept C2779849746 @default.
- W1988394499 hasConcept C2780151546 @default.
- W1988394499 hasConcept C2781448156 @default.
- W1988394499 hasConcept C42360764 @default.
- W1988394499 hasConcept C521977710 @default.
- W1988394499 hasConcept C71924100 @default.
- W1988394499 hasConcept C86803240 @default.
- W1988394499 hasConceptScore W1988394499C112613896 @default.
- W1988394499 hasConceptScore W1988394499C115537861 @default.
- W1988394499 hasConceptScore W1988394499C12554922 @default.
- W1988394499 hasConceptScore W1988394499C127413603 @default.
- W1988394499 hasConceptScore W1988394499C136229726 @default.
- W1988394499 hasConceptScore W1988394499C159985019 @default.
- W1988394499 hasConceptScore W1988394499C171250308 @default.