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• W1988399053 abstract "Glycerol can be biologically converted to 1,3-propanediol (1,3-PD), a key raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers. 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-PD synthesis biologically pathway. In present study, the dhaT gene encoding PDOR was cloned from Citrobacter freundii and overexpressed in E. coli BL21. The recombinant enzyme was purified by nickel-chelate chromatography combined with gel filtration to study the enzymatic characterization. Specific activity of recombinant PDOR was 55.2 U/mg. K m and V max values were 8.9 mM, 40.2 U/mg respectively. Holoenzyme of PDOR maybe a decamer of which a monomer has a molecular mass of 43 kDa. This research may form a basis for the future work on biological synthesis of 1, 3-PD." @default.
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• W1988399053 date "2014-12-01" @default.
• W1988399053 modified "2023-09-26" @default.
• W1988399053 title "Overexpression and Characterization of the Gene Encoding 1,3-Propanediol Oxidoreductase of <i>Citrobacter freundii</i>" @default.
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• W1988399053 doi "https://doi.org/10.4028/www.scientific.net/kem.636.121" @default.
• W1988399053 hasPublicationYear "2014" @default.
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