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- W1988428427 abstract "To identify ovarian autoantigens associated with ovarian autoantibodies.Hypothesis-generating prospective study.Urban infertility referral centers and academic research institution.Seventy-four patients with infertility, 19 patients with premature ovarian failure (POF), and 16 healthy control women.None.Identification of autoantigens.To identify major antigens for ovarian autoimmunity, sera from 74 women with unexplained infertility were screened for ovarian autoantibodies (AOAs) by immunoassay and one-dimensional Western blot. The majority of sera had immunoreactions at 50-56 kDa. Six representative positive infertility sera were used to identify antigens between 40 and 60 kD by two-dimensional Western blot and mass spectrometry. Antigens included aldehyde (retinal) dehydrogenases (ALDH1A1, ALDH1A2, and ALDH7A1), protein disulfide isomerase A3, vimentin, α-enolase, phosphoglycerate dehydrogenase, and selenium-binding protein 1 (SBP1). Sixty percent (24 out of 40) of infertility and POF sera were positive for recombinant ALDH1A1, SBP1, or enolase; 80.7% (21 out of 26) of AOA-positive sera had antibodies to one or more of the three antigens, and only 7% (1 out of 14) of AOA-negative sera had antibodies to recombinant proteins.ALDH1A1 and SBP1 are unique to ovarian autoimmunity associated with infertility and POF, and may provide the basis for specific tests to identify patients with ovarian autoimmunity." @default.
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- W1988428427 date "2010-12-01" @default.
- W1988428427 modified "2023-10-06" @default.
- W1988428427 title "Autoantigens in ovarian autoimmunity associated with unexplained infertility and premature ovarian failure" @default.
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- W1988428427 doi "https://doi.org/10.1016/j.fertnstert.2010.04.012" @default.
- W1988428427 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2948062" @default.
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