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• W1988458046 abstract "Spontaneous contractions of the rat portal vein were potentiated in magnitude by phenylephrine, cyclopiazonic acid, ryanodine or caffeine. All these drugs can deplete Ca2+ from intracellular stores, which stimulates store-operated cation entry in some tissues. The possibility that depletion of Ca2+ from intracellular stores potentiates the spontaneous contractions was therefore investigated using functional experiments. Phenylephrine or cyclopiazonic acid was added to tissues in Ca2+-free Krebs solution, followed by a 30-min washout. After addition of extracellular Ca2+, the spontaneous contractions were potentiated. This showed the stimulus for potentiating the contractions remained so long as intracellular Ca2+ stores were depleted. Following phenylephrine washout in normal Krebs solution, potentiation of the spontaneous contractions was attenuated with time. This attenuation was abolished by the protein kinase C inhibitor calphostin C. These results show depletion of Ca2+ from intracellular stores potentiates spontaneous contractions of the portal vein. Protein kinase C may inhibit this mechanism." @default.
• W1988458046 created "2016-06-24" @default.
• W1988458046 creator A5058994056 @default.
• W1988458046 date "2004-08-01" @default.
• W1988458046 modified "2023-09-27" @default.
• W1988458046 title "Depletion of Ca2+ from intracellular stores potentiates spontaneous contractions of the rat portal vein" @default.
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• W1988458046 doi "https://doi.org/10.1016/j.ejphar.2004.05.032" @default.
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