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- W1988482525 abstract "Abstract Ts + recombinant viruses were isolated from cells mixedly infected, at the nonpermissive temperature, with the intact DNA of HSV-1 ts mutants and unseparated fragments of wildtype HSV-2 DNA. Restriction enzyme analysis of the genomes of these intertypic recombinants enabled the cross-over points to be mapped. Recombinants generated with a particular ts mutant DNA contained relatively small overlapping insertions of HSV-2 DNA which enabled accurate physical mapping of the original ts lesion. Five ts mutations were mapped using this approach, and in each case, the results confirmed those previously obtained by marker rescue with isolated restriction endonuclease fragments of wild-type HSV-1 DNA." @default.
- W1988482525 created "2016-06-24" @default.
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- W1988482525 date "1978-10-01" @default.
- W1988482525 modified "2023-10-18" @default.
- W1988482525 title "Physical mapping of temperature-sensitive mutations of herpes simplex virus type 1 by intertypic marker rescue" @default.
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- W1988482525 doi "https://doi.org/10.1016/0042-6822(78)90327-6" @default.
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