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- W1988526868 abstract "A deconvolution of the difference spectra (in the near-ultraviolet and visible regions) associated with the successive oxidation steps of the oxygen-evolving complex is presented. The experimental procedure was designed to eliminate any contribution from the secondary quinone acceptor QB and to obtain contrasted sequences with respect to the initial distribution of the S0/S1 states. Both conditions were obtained by using various preillumination procedures in the presence of a low concentration of carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP). This substance accelerates the deactivation of states S2 and S3 without affecting the redox state of the two-electron gate QB, so that the steady-state equipartition of QB and Q−B reached through a continuous preillumination is left undisturbed during the deactivation of the S-states. Only a small absorption change is associated with the S0 → S1 transition in the spectral region investigated. The spectrum of the S1 → S2 transition consists of a broad band peaking around 315 nm and a chlorophyll a bandshift in the blue region. The bandshift is mostly absent from the spectrum of S2 → S3 which also differs slightly from that of S1 → S2 in the ultraviolet with a smaller band peaking around 305 nm. These results are consistent with - but do not ascertain - a manganese oxidation Mn (III → IV) occurring on the two latter steps. The weakness of the S0 → S1 spectrum may suggest that manganese is not involved at this step, although an Mn (II → III) oxidation cannot be ruled out. An additional spectral component occurs upon the first flash of the sequences. The spectrum of this slow turnover signal is presented and discussed." @default.
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- W1988526868 date "1987-10-01" @default.
- W1988526868 modified "2023-09-26" @default.
- W1988526868 title "Optical-difference spectra of the S-state transitions in the photosynthetic oxygen-evolving complex" @default.
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- W1988526868 doi "https://doi.org/10.1016/0005-2728(87)90215-5" @default.
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