Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988569869> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1988569869 endingPage "170" @default.
- W1988569869 startingPage "167" @default.
- W1988569869 abstract "Objectives: Some biochemical characteristics of the human leukocyte and fibroblast β-galactosidase were studied. Design and Methods: Leukocyte and fibroblast enzyme activity was determined fluorometricaly using 4-methylumbelliferyl-β-D-galactoside as artificial substrate. Optimum pH, Km, Vmax and thermostability of the enzyme at 42 °C were determined. Results: The leukocyte and fibroblast enzyme has an optimum pH at 4.2, which is in agreement with the lysosomal origin of the enzyme. The Km of the enzyme was 0.62 in leukocytes and 0.67 in fibroblasts, and Vmax was 289.9 nmol/h/mg of protein and 1779.2 nmol/h/mg of protein in the two tissues, respectively. When fibroblast or leukocyte β-galactosidase was pre-incubated at 42 °C, it did not retain its activity because the residual activity after 80 minutes of pre-incubation at this temperature was lower than 30% of the initial activity both in leukocytes and fibroblasts. Conclusions: This was the first study of Km, Vmax and thermostability of β-galactosidase performed on leukocytes and provided data for a better characterization of the enzyme β-galactosidase, allowing the improvement of the analytical conditions." @default.
- W1988569869 created "2016-06-24" @default.
- W1988569869 creator A5003213652 @default.
- W1988569869 creator A5055892112 @default.
- W1988569869 creator A5056733404 @default.
- W1988569869 creator A5060560862 @default.
- W1988569869 creator A5084680044 @default.
- W1988569869 creator A5089564343 @default.
- W1988569869 date "1999-04-01" @default.
- W1988569869 modified "2023-09-27" @default.
- W1988569869 title "Biochemical studies on leukocyte and fibroblast human β-galactosidase" @default.
- W1988569869 cites W1775749144 @default.
- W1988569869 cites W1965667194 @default.
- W1988569869 cites W1976921401 @default.
- W1988569869 cites W1996003640 @default.
- W1988569869 cites W2031827755 @default.
- W1988569869 cites W2054979333 @default.
- W1988569869 cites W2065323619 @default.
- W1988569869 cites W2075792282 @default.
- W1988569869 cites W2092821521 @default.
- W1988569869 cites W2097514332 @default.
- W1988569869 cites W2257840380 @default.
- W1988569869 cites W2587147763 @default.
- W1988569869 cites W3017274801 @default.
- W1988569869 doi "https://doi.org/10.1016/s0009-9120(98)00107-6" @default.
- W1988569869 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10383075" @default.
- W1988569869 hasPublicationYear "1999" @default.
- W1988569869 type Work @default.
- W1988569869 sameAs 1988569869 @default.
- W1988569869 citedByCount "7" @default.
- W1988569869 countsByYear W19885698692014 @default.
- W1988569869 countsByYear W19885698692016 @default.
- W1988569869 crossrefType "journal-article" @default.
- W1988569869 hasAuthorship W1988569869A5003213652 @default.
- W1988569869 hasAuthorship W1988569869A5055892112 @default.
- W1988569869 hasAuthorship W1988569869A5056733404 @default.
- W1988569869 hasAuthorship W1988569869A5060560862 @default.
- W1988569869 hasAuthorship W1988569869A5084680044 @default.
- W1988569869 hasAuthorship W1988569869A5089564343 @default.
- W1988569869 hasConcept C153911025 @default.
- W1988569869 hasConcept C181199279 @default.
- W1988569869 hasConcept C185592680 @default.
- W1988569869 hasConcept C18903297 @default.
- W1988569869 hasConcept C202751555 @default.
- W1988569869 hasConcept C25642318 @default.
- W1988569869 hasConcept C2777289219 @default.
- W1988569869 hasConcept C2780381497 @default.
- W1988569869 hasConcept C2781216036 @default.
- W1988569869 hasConcept C28212737 @default.
- W1988569869 hasConcept C55493867 @default.
- W1988569869 hasConcept C86803240 @default.
- W1988569869 hasConceptScore W1988569869C153911025 @default.
- W1988569869 hasConceptScore W1988569869C181199279 @default.
- W1988569869 hasConceptScore W1988569869C185592680 @default.
- W1988569869 hasConceptScore W1988569869C18903297 @default.
- W1988569869 hasConceptScore W1988569869C202751555 @default.
- W1988569869 hasConceptScore W1988569869C25642318 @default.
- W1988569869 hasConceptScore W1988569869C2777289219 @default.
- W1988569869 hasConceptScore W1988569869C2780381497 @default.
- W1988569869 hasConceptScore W1988569869C2781216036 @default.
- W1988569869 hasConceptScore W1988569869C28212737 @default.
- W1988569869 hasConceptScore W1988569869C55493867 @default.
- W1988569869 hasConceptScore W1988569869C86803240 @default.
- W1988569869 hasIssue "3" @default.
- W1988569869 hasLocation W19885698691 @default.
- W1988569869 hasLocation W19885698692 @default.
- W1988569869 hasOpenAccess W1988569869 @default.
- W1988569869 hasPrimaryLocation W19885698691 @default.
- W1988569869 hasRelatedWork W1633704547 @default.
- W1988569869 hasRelatedWork W1988569869 @default.
- W1988569869 hasRelatedWork W2013201200 @default.
- W1988569869 hasRelatedWork W2014169934 @default.
- W1988569869 hasRelatedWork W2046196755 @default.
- W1988569869 hasRelatedWork W2064127850 @default.
- W1988569869 hasRelatedWork W2111001014 @default.
- W1988569869 hasRelatedWork W2124499287 @default.
- W1988569869 hasRelatedWork W3007517610 @default.
- W1988569869 hasRelatedWork W3217421314 @default.
- W1988569869 hasVolume "32" @default.
- W1988569869 isParatext "false" @default.
- W1988569869 isRetracted "false" @default.
- W1988569869 magId "1988569869" @default.
- W1988569869 workType "article" @default.