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• W1988578210 abstract "Embryos recovered from sows on Days 9–13 of pregnancy (Day 0 = first day of estrus) exhibited saturable and time-dependent specific binding of 125I-epidermal growth factor (EGF). The specific binding (pg/mg protein) was greater (P < 0.001) for Day 13 elongated conceptuses than for conceptuses of earlier stages. Scatchard analyses showed two classes of binding sites (Kd = 7.0 ± 2.6 × 10−11 M, Bmax = 6.2 ± 1.4 fmol/mg protein and Kd = 3.4 ± 0.2 × 10−8 M, Bmax = 420 ± 80 fmol/mg protein). The EGF receptor in Day 13 conceptus membranes is a 170-kDa protein and was phosphorylated in the presence of EGF and adenosine triphosphate. EGF stimulated protein tyrosine kinase activity about 1.6-fold over basal levels. The results show that the preimplantation pig conceptus possesses EGF-binding sites with the properties of functional EGF-receptors." @default.
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• W1988578210 date "1992-06-01" @default.
• W1988578210 modified "2023-09-27" @default.
• W1988578210 title "Characterization of the epidermal growth factor receptor in preimplantation pig conceptuses" @default.
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• W1988578210 doi "https://doi.org/10.1016/0012-1606(92)90199-q" @default.
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