Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988623603> ?p ?o ?g. }
- W1988623603 endingPage "386" @default.
- W1988623603 startingPage "377" @default.
- W1988623603 abstract "A major barrier in the discovery of new secondary metabolites from microorganisms is the difficulty of distinguishing the minor fraction of productive cultures from the majority of unproductive cultures and growth conditions. In this study, a rapid, direct-infusion electrospray mass spectrometry (ES-MS) technique was used to identify chemical differences that occurred in the expression of secondary metabolites by 44 actinomycetes cultivated under six different fermentation conditions. Samples from actinomycete fermentations were prepared by solid-phase extraction, analyzed by ES-MS, and ranked according to a chemical productivity index based on the total number and relative intensity of ions present in each sample. The actinomycete cultures were tested for chemical productivity following treatments that included nutritional manipulations, autoregulator additions, and different agitation speeds and incubation temperatures. Evaluation of the ES-MS data from submerged and solid-state fermentations by paired t test analyses showed that solid-state growth significantly altered the chemical profiles of extracts from 75% of the actinomycetes evaluated. Parallel analysis of the same extracts by high-performance liquid chromatography-ES-MS-evaporative light scattering showed that the chemical differences detected by the ES-MS method were associated with growth condition-dependent changes in the yield of secondary metabolites. Our results indicate that the high-throughput ES-MS method is useful for identification of fermentation conditions that enhance expression of secondary metabolites from actinomycetes." @default.
- W1988623603 created "2016-06-24" @default.
- W1988623603 creator A5046881448 @default.
- W1988623603 creator A5052240068 @default.
- W1988623603 creator A5086799112 @default.
- W1988623603 date "2001-01-01" @default.
- W1988623603 modified "2023-09-23" @default.
- W1988623603 title "Use of Direct-Infusion Electrospray Mass Spectrometry To Guide Empirical Development of Improved Conditions for Expression of Secondary Metabolites from Actinomycetes" @default.
- W1988623603 cites W1031389965 @default.
- W1988623603 cites W1428290608 @default.
- W1988623603 cites W1496282049 @default.
- W1988623603 cites W1586058441 @default.
- W1988623603 cites W1967114014 @default.
- W1988623603 cites W1979142138 @default.
- W1988623603 cites W1983592663 @default.
- W1988623603 cites W2000126181 @default.
- W1988623603 cites W2001892476 @default.
- W1988623603 cites W2012465603 @default.
- W1988623603 cites W2017996336 @default.
- W1988623603 cites W2018990299 @default.
- W1988623603 cites W2023532185 @default.
- W1988623603 cites W2026096991 @default.
- W1988623603 cites W2040228452 @default.
- W1988623603 cites W2040985535 @default.
- W1988623603 cites W2042053478 @default.
- W1988623603 cites W2052463523 @default.
- W1988623603 cites W2063063617 @default.
- W1988623603 cites W2066076559 @default.
- W1988623603 cites W2074284463 @default.
- W1988623603 cites W2077348658 @default.
- W1988623603 cites W2087332559 @default.
- W1988623603 cites W2096563946 @default.
- W1988623603 cites W2100233093 @default.
- W1988623603 cites W2122432523 @default.
- W1988623603 cites W2127240188 @default.
- W1988623603 cites W2134555745 @default.
- W1988623603 cites W2138011534 @default.
- W1988623603 cites W2151988999 @default.
- W1988623603 cites W2258949670 @default.
- W1988623603 cites W2505819545 @default.
- W1988623603 cites W2577313308 @default.
- W1988623603 cites W4256636637 @default.
- W1988623603 doi "https://doi.org/10.1128/aem.67.1.377-386.2001" @default.
- W1988623603 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/92589" @default.
- W1988623603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11133469" @default.
- W1988623603 hasPublicationYear "2001" @default.
- W1988623603 type Work @default.
- W1988623603 sameAs 1988623603 @default.
- W1988623603 citedByCount "34" @default.
- W1988623603 countsByYear W19886236032012 @default.
- W1988623603 countsByYear W19886236032013 @default.
- W1988623603 countsByYear W19886236032014 @default.
- W1988623603 countsByYear W19886236032019 @default.
- W1988623603 countsByYear W19886236032023 @default.
- W1988623603 crossrefType "journal-article" @default.
- W1988623603 hasAuthorship W1988623603A5046881448 @default.
- W1988623603 hasAuthorship W1988623603A5052240068 @default.
- W1988623603 hasAuthorship W1988623603A5086799112 @default.
- W1988623603 hasBestOaLocation W19886236031 @default.
- W1988623603 hasConcept C100544194 @default.
- W1988623603 hasConcept C134121241 @default.
- W1988623603 hasConcept C162356407 @default.
- W1988623603 hasConcept C181199279 @default.
- W1988623603 hasConcept C185592680 @default.
- W1988623603 hasConcept C191897082 @default.
- W1988623603 hasConcept C192562407 @default.
- W1988623603 hasConcept C194451485 @default.
- W1988623603 hasConcept C21565614 @default.
- W1988623603 hasConcept C2777157883 @default.
- W1988623603 hasConcept C2779942233 @default.
- W1988623603 hasConcept C31903555 @default.
- W1988623603 hasConcept C40684141 @default.
- W1988623603 hasConcept C43617362 @default.
- W1988623603 hasConcept C553450214 @default.
- W1988623603 hasConcept C55493867 @default.
- W1988623603 hasConcept C86803240 @default.
- W1988623603 hasConceptScore W1988623603C100544194 @default.
- W1988623603 hasConceptScore W1988623603C134121241 @default.
- W1988623603 hasConceptScore W1988623603C162356407 @default.
- W1988623603 hasConceptScore W1988623603C181199279 @default.
- W1988623603 hasConceptScore W1988623603C185592680 @default.
- W1988623603 hasConceptScore W1988623603C191897082 @default.
- W1988623603 hasConceptScore W1988623603C192562407 @default.
- W1988623603 hasConceptScore W1988623603C194451485 @default.
- W1988623603 hasConceptScore W1988623603C21565614 @default.
- W1988623603 hasConceptScore W1988623603C2777157883 @default.
- W1988623603 hasConceptScore W1988623603C2779942233 @default.
- W1988623603 hasConceptScore W1988623603C31903555 @default.
- W1988623603 hasConceptScore W1988623603C40684141 @default.
- W1988623603 hasConceptScore W1988623603C43617362 @default.
- W1988623603 hasConceptScore W1988623603C553450214 @default.
- W1988623603 hasConceptScore W1988623603C55493867 @default.
- W1988623603 hasConceptScore W1988623603C86803240 @default.
- W1988623603 hasIssue "1" @default.
- W1988623603 hasLocation W19886236031 @default.
- W1988623603 hasLocation W19886236032 @default.
- W1988623603 hasLocation W19886236033 @default.
- W1988623603 hasLocation W19886236034 @default.