FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1988779505> ?p ?o ?g. }

• W1988779505 endingPage "461" @default.
• W1988779505 startingPage "454" @default.
• W1988779505 abstract "The extended role of vascular endothelial growth factor (VEGF) in human pathophysiology led us to evaluate pre-analytical parameters possibly influencing its levels in peripheral blood and tissues. The effects on VEGF protein levels and mRNA expression were measured after storage delay (blood and tissue), use of different types of anticoagulants (blood), and after different numbers of freeze-thaw cycles (blood).Blood from healthy donors was sampled simultaneously in ethylene diamine tetraacetic acid (EDTA), acid citrate dextrose (ACD-A), hirudin, and serum separation tubes. For each anticoagulant, VEGF was measured by enzyme-linked immunosorbent assay (ELISA) with different conditions of delay at 4°C before centrifugation (2 h, 4 h, or 48 h) and of different numbers of freeze-thaw cycles (1, 2, and 10). The transcripts coding for the VEGF165 isoform were quantified in peripheral blood mononuclear cells by RT-PCR. Muscle biopsy samples were frozen with delays of 15, 30, or 60 min after surgery. VEGF expression was quantified on immunofluorescence stained slides.The period of storage and the number of freeze-thaw cycles correlated with an increase in the levels of circulating VEGF (for each anticoagulant but not for serum) and its expression in PBMCs. VEGF expression measured from muscle biopsy sections was higher with freezing delays, with a peak at 30 and 60 min as compared to 15 min.The most reliable conditions for measuring both circulating VEGF and its gene expression are to reduce time between blood collection and centrifugation, and to avoid multiple freeze-thaw cycles. Serum collection tubes with no additive and no separator were less sensitive to the pre-analytical variations analyzed in this study. Freezing delay had a significant influence on VEGF protein expression in tissue samples." @default.
• W1988779505 created "2016-06-24" @default.
• W1988779505 creator A5001870256 @default.
• W1988779505 creator A5011110394 @default.
• W1988779505 creator A5011472212 @default.
• W1988779505 creator A5029042149 @default.
• W1988779505 creator A5049879208 @default.
• W1988779505 creator A5055270857 @default.
• W1988779505 creator A5059969167 @default.
• W1988779505 creator A5061738622 @default.
• W1988779505 creator A5064671837 @default.
• W1988779505 creator A5065419751 @default.
• W1988779505 creator A5074894204 @default.
• W1988779505 creator A5075890923 @default.
• W1988779505 creator A5077354137 @default.
• W1988779505 date "2012-10-01" @default.
• W1988779505 modified "2023-10-18" @default.
• W1988779505 title "Influence of Pre-analytical Variables on VEGF Gene Expression and Circulating Protein Concentrations" @default.
• W1988779505 cites W1549455161 @default.
• W1988779505 cites W1561059299 @default.
• W1988779505 cites W1577577364 @default.
• W1988779505 cites W1965779501 @default.
• W1988779505 cites W1985712533 @default.
• W1988779505 cites W1990529279 @default.
• W1988779505 cites W2000552840 @default.
• W1988779505 cites W2003656890 @default.
• W1988779505 cites W2006369124 @default.
• W1988779505 cites W2010556151 @default.
• W1988779505 cites W2020242316 @default.
• W1988779505 cites W2020277172 @default.
• W1988779505 cites W2033789194 @default.
• W1988779505 cites W2041199873 @default.
• W1988779505 cites W2044412918 @default.
• W1988779505 cites W2054618471 @default.
• W1988779505 cites W2055868288 @default.
• W1988779505 cites W2058006228 @default.
• W1988779505 cites W2064739543 @default.
• W1988779505 cites W2065232910 @default.
• W1988779505 cites W2072143531 @default.
• W1988779505 cites W2074277198 @default.
• W1988779505 cites W2078588946 @default.
• W1988779505 cites W2081038296 @default.
• W1988779505 cites W2087911316 @default.
• W1988779505 cites W2092409918 @default.
• W1988779505 cites W2095893831 @default.
• W1988779505 cites W2097627055 @default.
• W1988779505 cites W2120264116 @default.
• W1988779505 cites W2122415594 @default.
• W1988779505 cites W2125791676 @default.
• W1988779505 cites W2137947798 @default.
• W1988779505 cites W2141046053 @default.
• W1988779505 cites W2149416256 @default.
• W1988779505 cites W2153357579 @default.
• W1988779505 cites W2166618443 @default.
• W1988779505 cites W2171475230 @default.
• W1988779505 cites W4237022134 @default.
• W1988779505 doi "https://doi.org/10.1089/bio.2012.0016" @default.
• W1988779505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24845047" @default.
• W1988779505 hasPublicationYear "2012" @default.
• W1988779505 type Work @default.
• W1988779505 sameAs 1988779505 @default.
• W1988779505 citedByCount "14" @default.
• W1988779505 countsByYear W19887795052013 @default.
• W1988779505 countsByYear W19887795052014 @default.
• W1988779505 countsByYear W19887795052015 @default.
• W1988779505 countsByYear W19887795052016 @default.
• W1988779505 countsByYear W19887795052018 @default.
• W1988779505 countsByYear W19887795052019 @default.
• W1988779505 countsByYear W19887795052020 @default.
• W1988779505 countsByYear W19887795052022 @default.
• W1988779505 crossrefType "journal-article" @default.
• W1988779505 hasAuthorship W1988779505A5001870256 @default.
• W1988779505 hasAuthorship W1988779505A5011110394 @default.
• W1988779505 hasAuthorship W1988779505A5011472212 @default.
• W1988779505 hasAuthorship W1988779505A5029042149 @default.
• W1988779505 hasAuthorship W1988779505A5049879208 @default.
• W1988779505 hasAuthorship W1988779505A5055270857 @default.
• W1988779505 hasAuthorship W1988779505A5059969167 @default.
• W1988779505 hasAuthorship W1988779505A5061738622 @default.
• W1988779505 hasAuthorship W1988779505A5064671837 @default.
• W1988779505 hasAuthorship W1988779505A5065419751 @default.
• W1988779505 hasAuthorship W1988779505A5074894204 @default.
• W1988779505 hasAuthorship W1988779505A5075890923 @default.
• W1988779505 hasAuthorship W1988779505A5077354137 @default.
• W1988779505 hasConcept C104317684 @default.
• W1988779505 hasConcept C126322002 @default.
• W1988779505 hasConcept C132946820 @default.
• W1988779505 hasConcept C137061746 @default.
• W1988779505 hasConcept C150194340 @default.
• W1988779505 hasConcept C153911025 @default.
• W1988779505 hasConcept C1621761 @default.
• W1988779505 hasConcept C16685009 @default.
• W1988779505 hasConcept C167734588 @default.
• W1988779505 hasConcept C185592680 @default.
• W1988779505 hasConcept C202751555 @default.
• W1988779505 hasConcept C203014093 @default.
• W1988779505 hasConcept C2777025900 @default.
• W1988779505 hasConcept C2777292125 @default.

• next