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- W1988799512 abstract "Abstract Centrifugation techniques that reportedly yield a specific surface active lipoprotein from pulmonary extracts were applied to selected fractions with the view to see if they contained serum type lipoproteins. The fractions were investigated with the aid of qualitative and quantitative methods. These included disc electrophoresis with specific staining for lipoprotein, and determination of phospholipid and protein content of the various Centrifugation fractions. Ultracentrifugation and disc electrophoresis, which revealed consistently purified serum lipoproteins as well as the lipoproteins in intact serum, did not show any such lipoprotein in pulmonary washings or lung homogenates. We cannot exclude however the presence of a special type of lipoprotein or lipid-protein aggregate which breaks into lipid and protein during Ultracentrifugation or disc electrophoresis." @default.
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- W1988799512 date "1971-06-01" @default.
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- W1988799512 title "Significance of methods for isolation and characterization of pulmonary surfactants" @default.
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- W1988799512 doi "https://doi.org/10.1016/0034-5687(71)90051-x" @default.
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